Methods for pre-processing cane to obtain enzymative hydrolysates with high sugar content

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Methods of cane pretreatment (grinding, hydrothermal treatment, treatment with acid or alkali solutions, organosolv, deep eutectic solvents) and their effect on its subsequent enzymatic hydrolysis by cellulases and hemicellulases complexes have been studied. Substrates with the highest reactivity were obtained by exposing the cane to a weakly alkaline deep eutectic solvent (DES) and an alkali solution. The depth of enzymatic hydrolysis of these pretreated substrates was 63 and 59%, and the degree of conversion of polysaccharides (cellulose and hemicellulose) into monosaccharides taking into account the yield of the substrate at the pre-processing stage was 60 and 34%, respectively. After pre-treatment of the cane with acid DES, water steam, water or water/organic solution of sulfuric acid the depth of enzymatic hydrolysis was 45, 25, 20 and 11%, and the degree of polysaccharide conversion was 26, 18, 13 and 10%, respectively. The industrial enzyme preparation Agrocell Plus with a predominant content of cellobiohydrolases and endoglucanases was most effective in hydrolyzing the dust fraction of cane, as well as cane pretreated with a solution of sulfuric acid or DES (acidic or alkaline). The industrial enzyme preparation Agroxil Plus, containing endoxylanase and cellobiohydrolases, was more effective in hydrolyzing cane after hydrothermal pretreatment or alkali solution. The results of the hydrolysis of cane pretreated with acidic or weakly alkaline DES under the action of individual (homogeneous) cellulases indicate that in both cases the key enzyme was cellobiohydrolase 1.

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M. Semenova

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

A. Rozhkova

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

D. Osipov

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

V. Telitsin

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

E. Rubtsova

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

E. Kondrat’eva

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

I. Vasil’eva

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

O. Morozova

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

A. Yaropolov

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

A. Sinitsyn

Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: amrojkova@yahoo.com
俄罗斯联邦, Moscow, 119071

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2. Fig. 1. Reed with different degrees of grinding in a knife mill: 1 – dust fraction, 2 – coarse fraction, 3 – original reed.

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3. Fig. 2. Depth of hydrolysis of coarse (1) and dust fractions (2) of crushed reed after 48 hours of treatment with FP Agrocell Plus (10 mg protein/g substrate) and F10 (1 mg protein/g substrate) at 50°C, pH 5.0. The abscissa shows the substrate concentration.

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4. Fig. 3. Concentration of BC after 48 h of hydrolysis of reed (40 g/l), pretreated with GER HCHL with MK (1) or MEA (2), under the action of purified enzymes (10 mg protein/g substrate) with the addition of β-glucosidase (1 mg protein/g substrate) at 50°C, pH 5.0: CBH – cellobiohydrolase, EG – endoglucanase, EC – endoxylanase.

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5. Fig. 4. Concentration of BC after 48 h of hydrolysis of cane (40 g/l) pretreated with GER XXl with MK (1, 2) or MEA (3, 4), under the action of mixtures of purified enzymes (4.5 mg total protein/g substrate) with the addition of β-glucosidase (0.45 mg protein/g substrate) at 50°C, pH 5.0: 1 and 3 – mixtures of CBG with EG; 1, 2 and 4 – with EC. The abscissa axis shows the percentage content of cellobiohydrolases in the mixtures relative to the total enzyme content.

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