Biofilms as a risk factor for infectious complications in surgery: a review of modern prevention and eradication methods

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Abstract

This review focuses on the analysis of bacterial biofilms as one of the most significant risk factors for the development and chronicity of infectious complications in surgery. Biofilms are structured communities of microorganisms encased in a protective exopolysaccharide matrix, which exhibit high resistance to standard antibiotic therapy and host immune factors through a combination of mechanisms, including limited diffusion of antibacterial agents, the presence of metabolically inactive persister cells, and active horizontal gene transfer of resistance determinants.

This review systematizes data on the clinical significance of biofilms in implant-associated infections, chronic wounds, peritonitis, and mediastinitis. Modern diagnostic methods are examined in detail, highlighting the limitations of traditional microbiological culturing techniques and the significant potential of imaging approaches (specifically confocal microscopy), molecular genetic methods, and the value of detecting specific biofilm biomarkers.

The section on prevention emphasizes the methods for modifying implant surfaces and the application of antimicrobial coatings during surgical procedures. Eradication strategies are analyzed from the perspective of a combined approach, encompassing radical surgical debridement of the infectious focus, physical methods (negative pressure wound therapy with instillation, ultrasonic cavitation), and contemporary pharmacotherapy principles that involve local and systemic antibiotic delivery. Promising innovative directions are highlighted separately, including the use of quorum-sensing inhibitors, bacteriophages producing depolymerases, enzymatic matrix degradation, electrochemical methods, and nanotechnological systems for targeted delivery of antibacterial agents.

We emphasize the necessity of developing multimodal strategies that integrate surgical, physical, and pharmacological methods for the effective control of biofilm-associated infections, which is expected to subsequently reduce the incidence of postoperative complications.

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Diana F. Sangova

Izhevsk State Medical University

Author for correspondence.
Email: punk.scientist.fast@gmail.com
ORCID iD: 0009-0007-1165-6581
Russian Federation, Izhevsk

Elizaveta R. Krutikova

Izhevsk State Medical University

Email: yelizaveta.krutikova03@mail.ru
ORCID iD: 0009-0009-6758-7583
Russian Federation, Izhevsk

Adelina I. Alyavetdinova

Izhevsk State Medical University

Email: ildarovna28@bk.ru
ORCID iD: 0009-0002-0267-7536
Russian Federation, Izhevsk

Ilsur Sh. Shaydullin

Izhevsk State Medical University

Email: ilsur5555555555440@mail.ru
ORCID iD: 0009-0008-0233-4246
Russian Federation, Izhevsk

Ilmir A. Valiyakhmetov

Izhevsk State Medical University

Email: ilmirv7@gmail.com
ORCID iD: 0009-0003-5974-2295
Russian Federation, Izhevsk

Danil L. Garifyanov

Izhevsk State Medical University

Email: danil.garifyanow@yandex.ru
ORCID iD: 0009-0003-2334-9176
Russian Federation, Izhevsk

Elina M. Sadykova

Izhevsk State Medical University

Email: elinoshka50@gmail.com
ORCID iD: 0009-0001-6032-9671
Russian Federation, Izhevsk

Roman O. Volodin

Voronezh State Medical University

Email: gelog552@gmail.com
ORCID iD: 0009-0001-6216-898X
Russian Federation, Voronezh

Vladimir V. Salamatin

Voronezh State Medical University

Email: voci4ka@yandex.ru
ORCID iD: 0009-0008-6364-9292
Russian Federation, Voronezh

Leyla V. Guseynova

Irkutsk State Medical University

Email: brodskayaaal@gmail.com
ORCID iD: 0009-0002-7570-2093
Russian Federation, Irkutsk

Margarita L. Lbova

Izhevsk State Medical University

Email: margo.lbova@mail.ru
ORCID iD: 0009-0000-2519-863X
Russian Federation, Izhevsk

Angelina A. Korneeva

Irkutsk State Medical University

Email: korneevaangel03@mail.ru
ORCID iD: 0009-0007-6750-1589
Russian Federation, Irkutsk

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2. Fig. 1. Visual model of biofilm stability and therapeutic targets. ABT – antibiotic therapy; EPS – extracellular polymeric substance (matrix); QS – quorum sensing.

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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
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