Russian MedicineRussian MedicineРоссийский медицинский журнал0869-21062412-9100Eco-Vector4254710.18821/0869-2106-2020-26-2-93-97Clinical pharmacology and pharmaceuticalsКлиническая фармакология и лекарственные средстваResearch ArticleUse of endogenous cholesterol and its metabolite as markers of cyp450 metabolic activityИспользование эндогенного холестерина и его метаболита как маркеров метаболической активности cyp450https://orcid.org/0000-0002-8232-6682SmirnovV. V.СмирновВалерий Валерьевич
Russian Federation

MD, PhD

к.ф.н.

vall@mail.mipt.ruhttps://orcid.org/0000-0003-3650-6014KrasnykhL. M.КрасныхЛюдмила Михайловна
Russian Federation

MD, PhD

к.б.н.

krasnyh@expmed.ruhttps://orcid.org/0000-0001-7940-1664VasilenkoG. F.ВасиленкоГалина Федоровна
Russian Federation

MD, PhD

к.б.н.

vaselek1945@list.ruhttps://orcid.org/0000-0001-8779-3573RamenskayaG. V.РаменскаяГалина Владиславовна
Russian Federation

MD, PhD, DSc, Professor

д.ф.н., профессор

ramenskaia@mail.ruhttps://orcid.org/0000-0002-7900-1078ChechaO. A.ЧечаОльга Александровна
Russian Federation

MD, PhD

к.б.н.

olga.tchecha@yandex.ruScientific Centre for Expert Evaluation of Medicinal ProductsФГБУ «Научный центр экспертизы средств медицинского применения» Минздрава России (ФГБУ «НЦЭСМП» Минздрава России)I.M. Sechenov First Moscow State Medical University (Sechenov University)ФГАОУ ВО «Первый Московский Государственный Медицинский Университет им. И.М. Сеченова19082020262939719082020Copyright ©; 2020, Eco-VectorCopyright ©; 2020, ООО "Эко-Вектор"2020Eco-VectorООО "Эко-Вектор"https://medjrf.com/0869-2106/article/view/42547

This article analyzes the application of the method for determining the activity of CYP3A4 by determining the concentration of endogenous cholesterol and its metabolite/4β-hydroxycholine in blood plasma. It also provides information on the convergence assessment of data obtained using alternative methods for determining the activity of the same CYP450 isoenzyme (CYP3A4) using different substrates: cholesterol and cortisol.

Статья посвящена анализу применения методики определения активности CYP3A4 с помощью определения концентрации в плазме крови эндогенного холестерина и его метаболита — 4β-гидроксихолестерина. Также в статье приведены данные по оценке сходимости данных, полученных при использовании альтернативных методик определения активности одного и того же изофермента CYP450 (CYP3A4) с помощью различных субстратов: холестерина и кортизола.

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