Biology BulletinBiology BulletinИзвестия Российской академии наук. Серия биологическая1026-34703034-5367The Russian Academy of Sciences64782910.31857/S1026347024060038ukxmgtBIOCHEMISTRYБИОХИМИЯResearch ArticleActivity of energy and carbohydrate metabolism enzymes in Rainbow Trout fingerlings (Oncorhynchus mykiss Walb.) when feeding two types of commercial feedАктивность ферментов энергетического и углеводного обмена у сеголеток радужной форели (Oncorhynchus mykiss Walb.) при кормлении двумя видами коммерческих кормовRodinМ. А.РодинМ. А.
Russian Federation

Institute of Biology

Институт биологии

mikhail.rodin.mr@yandex.ruKuznetsovaМ. V.КузнецоваМ. В.
Russian Federation

Institute of Biology

Институт биологии

mikhail.rodin.mr@yandex.ruKrupnovaМ. Yu.КрупноваМ. Ю.
Russian Federation

Institute of Biology

Институт биологии

mikhail.rodin.mr@yandex.ruKuritsynА. Е.КурицынА. Е.
Russian Federation

Institute of Biology

Институт биологии

mikhail.rodin.mr@yandex.ruMurzinaS. А.МурзинаС. А.
Russian Federation

Institute of Biology

Институт биологии

mikhail.rodin.mr@yandex.ruNemovaN. N.НемоваН. Н.
Russian Federation

Institute of Biology

Институт биологии

mikhail.rodin.mr@yandex.ruFederal Research Center “Karelian Scientific Center of the Russian Academy of Sciences”Федеральный исследовательский центр «Карельский научный центр РАН»26122024670571528012025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://medjrf.com/1026-3470/article/view/647829

We studied the activity of energy and carbohydrate metabolism enzymes (cytochrome c oxidase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, 1-glycerophosphate dehydrogenase, pyruvate kinase, aldolase) in the muscles and liver of rainbow trout of three size groups aged 5, 10 and 12 months under the influence of two types of commercial feeds with different composition. The levels of activity of the enzymes G6PDH, 1-GPDH and aldolase in the liver were significantly higher in fish from the “Feed №2” group. The identified differences in the activity of enzymes in the liver of fish suggest that feed №2 to a greater extent (compared to feed №1) promotes the use of carbohydrates in lipid biosynthesis. Differences in the activity of the enzymes COX, LDH, aldolase, G6PDH and 1-GPDH in the liver and muscles of fish depending on the month of sampling and belonging to the size group are most likely associated with changes in the metabolism of fish as their weight increases towards generative metabolism.

Исследовали активность ферментов энергетического и углеводного обмена (цитохром с оксидазы, лактатдегидрогеназы, глюкозо-6-фосфатдегидрогеназы, 1-глицерофосфатдегидрогеназы, пируваткиназы, альдолазы) в мышцах и печени радужной форели Oncorhynchus mykiss Walb. трех размерных групп (РГ) возрастом 5, 10 и 12 месяцев при влиянии двух видов коммерческих кормов с различающимся составом. Уровни активности ферментов Г6ФДГ, 1-ГФДГ и альдолазы в печени были достоверно выше у рыб из группы «Корм №2». Выявленные различия в активности ферментов в печени рыб позволяют предположить, что корм №2 в большей степени (по сравнению с кормом №1) способствует использованию углеводов в биосинтезе липидов. Различия в активности ферментов ЦО, ЛДГ, альдолазы, Г6ФДГ и 1-ГФДГ в печени и мышцах рыб в зависимости от месяца сбора проб и принадлежности к размерной группе связаны, вероятнее всего, с перестройками в метаболизме рыб по мере увеличения массы в сторону генеративного обмена.

Rainbow Troutenergy metabolismenzyme activityfeed compositionрадужная форельэнергетический обменактивность ферментовсостав кормаМинистерство науки и высшего образования Российской ФедерацииMinistry of Science and Higher Education of the Russian Federation075-01637-22-00Колб В. Г., Камышников В. С. Клиническая биохимия // Минск: Изд-во Беларусь. 1976. 311 с.Кочетов Г. А. Практическое руководство по энзимологии // М.: Высш. шк. 1980. 272 с.Озернюк Н. Д. Энергетический обмен в раннем онтогенезе рыб // М.: Наука. 1985. 175 с.Чурова М. В., Мещерякова О. В., Веселов А. Е., Немова Н. Н. Активность ферментов энергетического и углеводного обмена и уровень некоторых молекулярно-генетических показателей у молоди лосося (Salmo salar L.), различающейся возрастом и массой // Онтогенез. 2015. T. 46. № 5. P. 304–312. https://doi.org/10.1134/ S1062360415050021Чурова М. В., Мещерякова О. В., Немова Н. Н., Шатуновский М. И. Соотношение роста и некоторых биохимических показателей рыб на примере микижи Parasalmo mykiss Walb. // Известия РАН. Сер. Биол. 2010. № 3. С. 289–299.Barroso J. B., Garcia-Salguero L., Peragon J., de la Higuera M., Lupiañez J. A. Effects of long-term starvation on the NADPH production systems in several different tissues of rainbow trout (Oncorhynchus mykiss) // Paris: INRA. 1993. P. 333–338.Bastrop R., Jurss K., Wacke R. Biochemical parameters as a measure of food availability and growth in immature rainbow trout (Oncorhynchus mykiss) // Comparative Biochemistry and Physiology. 1992. V. 102. P. 151–161. https://doi.org/10.1016/0300-9629(92)90028-OBoeuf G., Le Bail P. Y. Does light have an influence on fish growth? // Aquaculture. 1999. V. 177. № 1–4. P. 129–152. https://doi.org/10.1016/S0044-8486(99)00074-5Bradford M. M. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding // Anal. Biochem. 1976. V. 72. № 1–2. P. 248–254. https://doi.org/10.1016/0003-2697(76)90527-3Bücher T., Pfleiderer G. Pyruvate kinase from muscle // Methods in Enzymology. 1955. V. 1. P. 345–440.Burness G. P., Leary S. C., Hochachka P. W., Moyes C. D. Allometric scaling of RNA, DNA, and enzyme levels in fish muscle // Am. J. Physiol. 1999. V. 277. P. R1164–R1170. https://doi.org/10.1152/ajpregu.1999.277.4.R1164Ellerby D. J., Altringham J. D. Spatial variation in fast muscle function of the rainbow trout Oncorhynchus mykiss during fast-starts and sprinting // J. Exp. Biol. 2001. V. 204. P. 2239–2250. https://doi.org/10.1242/jeb.204.13.2239Enyu Y.-L., Shu-Chien A.C. Proteomics analysis of mitochondrial extract from liver of female zebrafish undergoing starvation and refeeding // Aquacult. Nutr. 2011. V. 17. № 2. P. e413–e423. https://doi.org/10.1111/j.1365-2095.2010.00776.xGauthier C., Campbell P., Couture P. Physiological correlates of growth and condition in the yellow perch (Perca flavescens) // Comparative Biochemistry and Physiology. Part A. 2008. V. 151. P. 526–532. https://doi.org/10.1016/j.cbpa.2008.07.010Harmon J. S., Sheridan M. A. Glucose-stimulated lipolysis in rainbow trout (Oncorhynchus mykiss) liver // J. Fish Physiol. Biochem. 1992. V. 10. P. 189–199. https://doi.org/10.1007/bf00004513Johansen K. A., Overturf K. Alterations in expression of genes associated with muscle metabolism and growth during nutritional restriction and refeeding in rainbow trout // Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2006. V. 144. P. 119–127. https://doi.org/10.1016/j.cbpb.2006.02.001Konradt J., Braunbeck T. Alterations of selected metabolic enzymes in fish following longterm exposure to contaminated streams // J. Aquat. Ecosystem Stress Recovery. 2001. V. 8. P. 299–318. https://doi.org/10.1023/a:1012928914322Kumar V., Sahu N. P., Pal A. K., Kumar S., Sinha A. K., Ranjan J., Baruah K. Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non‐gelatinized starch diet // Fish Physiology and Biochemistry. 2010. V. 36. P. 491–499. https://doi.org/10.1007/s10695-009-9319-5Llewellyn L., Sweeney G. E., Ramsurn V. P., Rogers S. A., Wigham T. Cloning and unusual expression profile of the aldolase B gene from Atlantic salmon // BBA Gene Structure and Expression. 1998. V. 1443. № 3. P. 375–380. https://doi.org/10.1016/S0167-4781(98)00229-2Meton I., Mediavilla D., Caseras A., Canto E., Fernandez F., Baanante I. V. Effect of diet composition and ration size on key enzyme activities of glycolysis-gluconeogenesis, the pentose phosphate pathway and amino acid metabolism in liver of gilthead sea bream (Sparus aurata) // Br. J. Nutr. 1999. V. 82. P. 223–232. https://doi.org/10.1017/S0007114599001403Peragon J., Barroso J. B., Garcia‐Salguero L., de la Higuera M., Lupianez J. A. Carbohydrates affect protein‐turnover rates, growth, and nucleic acid content in the white muscle of rainbow trout (Oncorhynchus mykiss) // Aquaculture. 1999. V. 179. P. 425–437. https://doi.org/10.1016/S0044-8486(99)00176-3Small B. C., Soares J. H.Jr. Effect of dietary carbohydrate on growth, glucose tolerance and liver composition of juveniles striped bass // North American Journal of Aquaculture. 1999. V. 61. P. 286–292. https://doi.org/10.1577/1548-8454(1999)061%3C0286:EODCOG%3E2.0.CO;2Smith L. Spectrophotometric assay of cytochrome C oxidase // Methods in Biochem. Analysis. 1955. V. 2. P. 427–434. https://doi.org/10.1002/9780470110188.ch13Soengas J. L., Sanmartin B., Barciela P., Aldegunde M., Rozas G. Changes in carbohydrate metabolism related to the onset of ovarian recrudescence in domesticated rainbow trout (Oncorhynchus mykiss) // Comp. Bioch. and Physiol. Part A: Physiology. 1993. V. 105. P. 293–301. https://doi.org/10.1016/0300-9629(93)90211-LSomero G. N., Childress J. J. A violation of the metabolism-size scaling paradigm: activities of glycolytic enzymes in muscle increase in larger size fish // Physiol. Zool. 1980. V. 53. № 3. P. 322–337. https://doi.org/10.1086/physzool.53.3.30155794Talukdar A., Kumar S., Varghese T., Jain K. K., Sahu N. P., Sahoo S. Feeding gelatinized carbohydrate in the diets of magur, Clarias batrachus (Linnaeus, 1758): Effects on growth performance, enzyme activities and expression of muscle regulatory factors // Aquaculture Research. 2019. V. 50. P. 765–777. https://doi.org/10.1111/are.13933Tian W. N., Braunstein L. D., Pang J., Stuhlmeier K. M., Xi Q. C., Tian X., Stanton R. C. Importance of glucose-6-phosphate dehydrogenase activity for cell growth // J. Biol. Chem. 1998. V. 273. P. 10609–10617. https://doi.org/10.1074/jbc.273.17.10609Treberg J. R., Lewis J. M., Driedzic W. R. Comparison of liver enzymes in osmerid fishes: key differences between a glycerol accumulating species, rainbow smelt (Osmerus mordax), and a species that does not accumulate glycerol, capelin (Mallotus villosus) // Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2002. V. 132. P. 433–438. https://doi.org/10.1016/S1095-6433(02)00083-1Yengkokpam S., Sahu N. P., Pal A. K., Mukherjee S. C., Debnath D. Gelatinized carbohydrates in the diet of Catla catla fingerlings: effect of levels and sources on nutrient utilization, body composition and tissue enzyme activities // Asian‐Australasian Journal of Animal Sciences. 2007. V. 20. № 1. P. 89–99. https://doi.org/10.5713/ajas.2007.89