Biology BulletinBiology BulletinИзвестия Российской академии наук. Серия биологическая1026-34703034-5367The Russian Academy of Sciences64783510.31857/S1026347024060064ukprkcBOTANYБОТАНИКАResearch ArticleAristolochia of Siphisia section in vitroAristolochia секции Siphisia in vitroNakonechnayaО. V.НаконечнаяО. В.
Russian Federation
markelova@biosoil.ruGafitskayaI. V.ГафицкаяИ. В.
Russian Federation
markelova@biosoil.ruYusupovaЕ. P.ЮсуповаЕ. П.
Russian Federation
markelova@biosoil.ruFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of SciencesФедеральный научный центр биоразнообразия наземной биоты Восточной Азии ДВО РАН26122024673574228012025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://medjrf.com/1026-3470/article/view/647835

The results of the study represent an optimization protocol for clonal micropropagation of 4 species of the genus Aristolochia, Siphisia section. Plants of these species are rare, endemic and medicinal plants. 6-Benzylaminopurine (BAP) (0.5, 1.0, 1.2 and 1.4 mg/L) and indolylbutyric acid (IBA) (0.5 and 1.0 mg/L) were used in this work. The use of subcultivation with different nutrient media (MS supplemented with 0.5 mg/l BAP at the propagation stage and ½ WPM with the addition of 0.5 mg/l IBA at the rooting stage) contributes to the mass production of microplants. The maximum values of the microshoot height for each species were detected on the medium with 0.5 mg/l BAP. Explants of A. macrophylla and A. manshuriensis were characterized with the greatest reproduction coefficient (34 pcs.); while A. californica explants displayed the least coefficient (18.6 pcs.). The difference is associated with the activation of a different number of buds in each species.

Результаты исследования представляют собой оптимизацию методики по клональному микроразмножению 4 видов рода Aristolochia секции Siphisia – редких, эндемичных и лекарственных растений. В работе использованы 6-бензиламинопурин (БАП) (0.5, 1.0, 1.2 и 1.4 мг/л) и индолилмасляная кислота (ИМК) (0.5 и 1.0 мг/л). Субкультивирование на разных питательных средах (МС с добавлением 0.5 мг/л БАП на этапе размножения и ½ ВПМ с добавлением 0.5 мг/л ИМК на этапе укоренения) способствовало массовому получению микрорастений. Максимальные значения высоты микропобегов каждого вида выявлены при использовании 0.5 мг/л БАП. Наибольшим потенциалом к размножению обладали экспланты A. macrophylla (коэффициент размножения равен 34 шт.) и A. manshuriensis (34 шт.), наименьшим – A. californica (18.6 шт.), что связано с активацией разного числа почек у каждого вида.

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