Evaluation of the cytotoxic combined effect of selenium and copper oxide nanoparticles in an acute experiment on rats

Cover Page

Cite item

Full Text

Abstract

Introduction. In the scientific literature known to us, there are no experimental data on the combined human health effect of nanoparticles of selenium and copper oxides, the exposure to which is feasible in metallurgy.

Materials and methods. The cytotoxic effect was modelled on outbred female rats by a single intratracheal instillation of suspended nanoparticles of selenium and copper oxides at a concentration of 0.25 mg/ml. Cytological and biochemical parameters of the bronchoalveolar lavage fluid were evaluated 24 hours after the administration of the suspension.

Results. The response of the lower airways to the combined exposure to SeO and CuO nanoparticles was more pronounced than that to the exposure to either of them, thus indicating its higher cytotoxicity as judged by cytological and biochemical parameters of the bronchoalveolar lavage fluid. The combined cytotoxic effect of SeO and CuO nanoparticles was characterized by typological diversity. According to the overwhelming number of the parameters studied, the additive nature of the combined effect of high exposure doses of SeO and CuO nanoparticles was demonstrated.

Limitations. The research was limited to the study of the main indicators of cytotoxic effects.

Conclusion. To avoid underestimation of the cumulative health risk for workers in the chemical and slime shops of copper smelters, it is important to take into consideration the additive nature of the combined effect of toxicants under study.

Compliance with ethical standards. The study was conducted in accordance with the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. The study protocol was approved by the Local Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers (Minutes No. 2 of April 20, 2021).

Contribution:
Ryabova Yu.V. — research concept and design, data collection and processing, statistical analysis, manuscript preparation, and editing;
Tazhigulova A.V. — research concept and design, data collection and processing, statistical analysis.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version. 

Conflict of interest. The authors declare no conflict of interest. 

Acknowledgement. The study had no sponsorship. 

Received: October 27, 2022 / Accepted: December 8, 2022 / Published: January 12, 2023

About the authors

Yuliya V. Ryabova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Author for correspondence.
Email: ryabovaiuvl@gmail.com
ORCID iD: 0000-0003-2677-0479

Research assistant, Laboratory of Scientific Bases for Biological Prophylaxis, Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Yekaterinburg, 620014, Russian Federation.

e-mail: ryabova@ymrc.ru

Russian Federation

Anastasia V. Tazhigulova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0001-9384-8550
Russian Federation

References

  1. Naboychenko C.S., ed. Selenium and Tellurium Production at Uralelectromed OJSC [Proizvodstvo selena i tellura na OAO «Uralelektromed’»: uchebnoe posobie]. Ekaterinburg; 2015. (in Russian)
  2. Lyapishchev Yu.B. Up-to-date processing of electrolytic copper refinery slimes. Zapiski Gornogo instituta. 2006; (2): 245–7. (in Russian)
  3. Madar’ I.I. Hydrometallurgical extraction of selenium from products of extraction processing of washing acid of copper production: Diss. St. Petersburg; 2015. (in Russian)
  4. Gurvich V.B., Katsnel’son B.A., Ruzakov V.O., Privalova L.I., Bushueva T.V. Biochemical effects in workers exposed to copper refinery nanoparticle-containing aerosols. In: Proceedings of the International Conference «Topics of Current Hygienic Importance in Nanotoxicology: Theoretical Premises, Hazards Identification and Ways of Their Attenuation» [Aktual’nye gigienicheskie aspekty nanotoksikologii: teoreticheskie osnovy, identifikatsiya opasnosti dlya zdorov’ya i puti ee snizheniya: Materialy mezhdunarodnoy konferentsii]. Yekaterinburg; 2016: 21–3. (in Russian)
  5. Privalova L.I., Katsnel’son B.A., Loginova N.V., Gurvich V.B., Shur V.Ya., Beykin Ya.B., et al. Cytological and biochemical characteristics of bronchoalveolar lavage fluid in rats after intratracheal instillation of copper oxide nano-scale particles. Toksikologicheskiy vestnik. 2014; (5): 8–15. (in Russian)
  6. Wu Y., Wang M., Luo S., Gu Y., Nie D., Xu Z., et al. Comparative toxic effects of manufactured nanoparticles and atmospheric particulate matter in human lung epithelial cells. Int. J. Environ. Res. Public Health. 2020; 18(1): 22. https://doi.org/10.3390/ijerph18010022
  7. Lin W., Huang Y.W., Zhou X.D., Ma Y. In vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicol. Appl. Pharmacol. 2006; 217(3): 252–9. https://doi.org/10.1016/j.taap.2006.10.004
  8. Liu N., Guan Y., Zhou C., Wang Y., Ma Z., Yao S. Pulmonary and systemic toxicity in a rat model of pulmonary alveolar proteinosis induced by indium-tin oxide nanoparticles. Int. J. Nanomedicine. 2022; 17: 713–31. https://doi.org/10.2147/IJN.S338955
  9. Guo C., Robertson S., Weber R.J.M., Buckley A., Warren J., Hodgson A., et al. Pulmonary toxicity of inhaled nano-sized cerium oxide aerosols in Sprague-Dawley rats. Nanotoxicol. 2019; 13(6): 733–50. https://doi.org/10.1080/17435390.2018.1554751
  10. He H., Zou Z., Wang B., Xu G., Chen C., Qin X., et al. Copper oxide nanoparticles induce oxidative DNA damage and cell death via copper ion-mediated P38 MAPK activation in vascular endothelial cells. Int. J. Nanomedicine. 2020; 15: 3291–302. https://doi.org/10.2147/IJN.S241157
  11. Alizadeh S.R., Ebrahimzadeh M.A. Characterization and anticancer activities of green synthesized CuO nanoparticles, a review. Anticancer Agents Med. Chem. 2021; 21(12): 1529–43. https://doi.org/10.2174/1871520620666201029111532
  12. Zou L., Cheng G., Xu C., Liu H., Wang Y., Li N., et al. Copper nanoparticles induce oxidative stress via the heme oxygenase 1 signaling pathway in vitro studies. Int. J. Nanomedicine. 2021; 16: 1565–73. https://doi.org/10.2147/IJN.S292319
  13. Zheng Z., Liu L., Zhou K., Ding L., Zeng J., Zhang W. Anti-oxidant and anti-endothelial dysfunctional properties of nano-selenium in vitro and in vivo of hyperhomocysteinemic rats. Int. J. Nanomedicine. 2020; 15: 4501–21. https://doi.org/10.2147/IJN.S255392
  14. Pi J., Yang F., Jin H., Huang X., Liu R., Yang P., et al. Selenium nanoparticles induced membrane bio-mechanical property changes in MCF-7 cells by disturbing membrane molecules and F-actin. Bioorg. Med. Chem. Lett. 2013; 23(23): 6296–303. https://doi.org/10.1016/j.bmcl.2013.09.078
  15. Huang G., Liu Z., He L., Luk K.H., Cheung S.T., Wong K.H., et al. Autophagy is an important action mode for functionalized selenium nanoparticles to exhibit anti-colorectal cancer activity. Biomater. Sci. 2018; 6(9): 2508–17. https://doi.org/10.1039/c8bm00670a
  16. Martínez-Esquivias F., Gutiérrez-Angulo M., Pérez-Larios A., Sánchez-Burgos J., Becerra-Ruiz J., Guzmán-Flores J.M. Anticancer activity of selenium nanoparticles in vitro studies. Anticancer Agents. Med. Chem. 2021; 22(9): 1658–73. https://doi.org/10.2174/1871520621666210910084216
  17. Kondaparthi P., Flora S.J.S., Naqvi S. Selenium nanoparticles: An insight on its pro-oxidant and antioxidant properties. Front. Nanosci. Nanotechnol. 2019; 6: 1–5. https://doi.org/10.15761/FNN.1000189
  18. Zhuang Y., Li L., Feng L., Wang S., Su H., Liu H., et al. Mitochondrion-targeted selenium nanoparticles enhance reactive oxygen species-mediated cell death. Nanoscale. 2020; 12(3): 1389–96. https://doi.org/10.1039/c9nr09039h
  19. Minigalieva I.A., Katsnelson B.A., Panov V.G., Privalova L.I., Varaksin A.N., Gurvich V.B., et al. In vivo toxicity of copper oxide, lead oxide and zinc oxide nanoparticles acting in different combinations and its attenuation with a complex of innocuous bio-protectors. Toxicol. 2017; 380: 72–93. https://doi.org/10.1016/j.tox.2017.02.007
  20. Sutunkova M.P., Privalova L.I., Ryabova Yu.V., Minigalieva I.A., Tazhigulova A.V., Labzova A.K., et al. Comparative assessment of the pulmonary effect in rats to a single intratracheal administration of selenium or copper oxide nanoparticles. Toksikologicheskiy vestnik. 2021; 29(6): 39–46. https://doi.org/10.36946/0869-7922-2021-29-6-39-46 (in Russian)
  21. Lebedev K.A., Ponyakina I.D. Immunogram in Clinical Practice: Introduction to Applied Immunology [Immunogramma v klinicheskoy praktike: Vvedenie v prikladnuyu immunologiyu]. Moscow: Nauka; 1990. (in Russian)
  22. Privalova L.I., Katsnelson B.A., Osipenko A.B., Yushkov B.N., Babushkina L.G. Response of a phagocyte cell system to products of macrophage breakdown as a probable mechanism of alveolar phagocytosis adaptation to deposition of particles of different cytotoxicity. Environ. Health Perspect. 1980; 35: 205–18. https://doi.org/10.1289/ehp.8035205
  23. Katsnelson B.A., Privalova L.I. Recruitment of phagocytizing cells into the respiratory tract as a response to the cytotoxic action of deposited particles. Environ. Health Perspect. 1984; 55: 313–25. https://doi.org/10.1289/ehp.8455313
  24. Privalova L.I., Katsnelson B.A., Yelnichnykh L.N. Some peculiarities of the pulmonary phagocytotic response: dust retention kinetics and silicosis development during long term exposure of rats to high quartz levels. Br. J. Ind. Med. 1987; 44(4): 228–35. https://doi.org/10.1136/oem.44.4.228
  25. Privalova L.I., Katsnelson B.A., Sharapova N.Y., Kislitsina N.S. On the relationship between activation and breakdown of macrophages in the pathogenesis of silicosis (an overview). Med. Lav. 1995; 86(6): 511–21.
  26. Ruenraroengsak P., Novak P., Berhanu D., Thorley A.J., Valsami-Jones E., Gorelik J., et al. Respiratory epithelial cytotoxicity and membrane damage (holes) caused by amine-modified nanoparticles. Nanotoxicol. 2012; 6(1): 94–108. https://doi.org/10.3109/17435390.2011.558643
  27. Cho W.S., Duffin R., Poland C.A., Duschl A., Oostingh G.J., Macnee W., et al. Differential pro-inflammatory effects of metal oxide nanoparticles and their soluble ions in vitro and in vivo; zinc and copper nanoparticles, but not their ions, recruit eosinophils to the lungs. Nanotoxicol. 2012; 6(1): 22–35. https://doi.org/10.3109/17435390.2011.552810
  28. Privalova L.I., Katsnelson B.A., Loginova N.V., Gurvich V.B., Shur V.Y., Valamina I.E., et al. Subchronic toxicity of copper oxide nanoparticles and its attenuation with the help of a combination of bioprotectors. Int. J. Mol. Sci. 2014; 15(7): 12379–406. https://doi.org/10.3390/ijms150712379

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2023 Ryabova Y.V., Tazhigulova A.V.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.