Evaluation of the quality composition of micromycetes in the air of the premises of stationary medical institutions

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

Introduction. Today, micromycetes are the most common pathogens found in residential and industrial premises and in the hospital environment. At the same time, mold fungi are the cause not only of allergic diseases but also of mycoses of various localization. Mold fungi are hazardous for patients in hospitals on long-term treatment and exposed to immunosuppression factors. Medical personnel who spent a long time in rooms with high contamination by micromycetes in the air can develop mycogenic sensitization. 

The aim of this work was to show the quantitative and qualitative composition of fungi surrounding the patient and the doctor in multidisciplinary hospitals. 

Materials and methods. With the help of a microbiological aspirator, air samples were taken in the premises of class B of surgical and therapeutic departments, followed by sowing on Saburo agar. The identified fungi were identified using classical methods and MALDI-ToF mass spectrometry using Microflex LT (Bruker). Results. In the course of the study, the assessment of fungal contamination of class B premises in treatment and prophylactic institutions was carried out to assess the qualitative and quantitative composition. Also, comparing the composition and number of micromycetes between the premises of departments of different profiles was carried out. A high level of contamination of micromycetes in medical premises of class B – 100% of cases was revealed in studies of objects of the hospital environment. The structure of the identified fungi was very diverse. In all air samples were isolated fungi genera Aspergillus, Penicillium, Mucor, Alternaria, and Ulcoladium. Spores of these fungi belonging to opportunistic or saprophytes are found in the air everywhere. Still, a significant increase in microbial load due to micromycetes can cause such typical allergic diseases as bronchial asthma, allergic skin dermatoses, and allergies of other locations. 

Conclusion. The study results revealed increased fungal contamination in healthcare facilities that require a more careful approach to compliance with the microclimate parameters.

Авторлар туралы

Dariya Tupikova

Samara State Medical University

Хат алмасуға жауапты Автор.
Email: noemail@neicon.ru
ORCID iD: 0000-0003-2813-7271
Ресей

Igor Berezin

Samara State Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-9370-7923
Ресей

Alexander Zhestkov

Samara State Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-3960-830X
Ресей

Artem Lyamin

Samara State Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-5905-1895
Ресей

Andrey Kozlov

Samara State Medical University

Email: kozlov.biochemistry@yandex.ru
ORCID iD: 0000-0001-9384-6854

Assistant of the Department of fundamental and clinical biochemistry with laboratory diagnostics, Samara State Medical University, Samara, 43099, Russian Federation.

e-mail: kozlov.biochemistry@yandex.ru

Ресей

Olga Sazonova

Samara State Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-4130-492X
Ресей

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© Tupikova D.S., Berezin I.I., Zhestkov A.V., Lyamin A.V., Kozlov A.V., Sazonova O.V., 2024


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