Evaluation of the quality composition of micromycetes in the air of the premises of stationary medical institutions

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Introduction. Today, micromycetes are the most common pathogens found in residential and industrial premises and in the hospital environment. At the same time, mold fungi are the cause not only of allergic diseases but also of mycoses of various localization. Mold fungi are hazardous for patients in hospitals on long-term treatment and exposed to immunosuppression factors. Medical personnel who spent a long time in rooms with high contamination by micromycetes in the air can develop mycogenic sensitization. 

The aim of this work was to show the quantitative and qualitative composition of fungi surrounding the patient and the doctor in multidisciplinary hospitals. 

Materials and methods. With the help of a microbiological aspirator, air samples were taken in the premises of class B of surgical and therapeutic departments, followed by sowing on Saburo agar. The identified fungi were identified using classical methods and MALDI-ToF mass spectrometry using Microflex LT (Bruker). Results. In the course of the study, the assessment of fungal contamination of class B premises in treatment and prophylactic institutions was carried out to assess the qualitative and quantitative composition. Also, comparing the composition and number of micromycetes between the premises of departments of different profiles was carried out. A high level of contamination of micromycetes in medical premises of class B – 100% of cases was revealed in studies of objects of the hospital environment. The structure of the identified fungi was very diverse. In all air samples were isolated fungi genera Aspergillus, Penicillium, Mucor, Alternaria, and Ulcoladium. Spores of these fungi belonging to opportunistic or saprophytes are found in the air everywhere. Still, a significant increase in microbial load due to micromycetes can cause such typical allergic diseases as bronchial asthma, allergic skin dermatoses, and allergies of other locations. 

Conclusion. The study results revealed increased fungal contamination in healthcare facilities that require a more careful approach to compliance with the microclimate parameters.

Sobre autores

Dariya Tupikova

Samara State Medical University

Autor responsável pela correspondência
Email: noemail@neicon.ru
ORCID ID: 0000-0003-2813-7271
Rússia

Igor Berezin

Samara State Medical University

Email: noemail@neicon.ru
ORCID ID: 0000-0002-9370-7923
Rússia

Alexander Zhestkov

Samara State Medical University

Email: noemail@neicon.ru
ORCID ID: 0000-0002-3960-830X
Rússia

Artem Lyamin

Samara State Medical University

Email: noemail@neicon.ru
ORCID ID: 0000-0002-5905-1895
Rússia

Andrey Kozlov

Samara State Medical University

Email: kozlov.biochemistry@yandex.ru
ORCID ID: 0000-0001-9384-6854

Assistant of the Department of fundamental and clinical biochemistry with laboratory diagnostics, Samara State Medical University, Samara, 43099, Russian Federation.

e-mail: kozlov.biochemistry@yandex.ru

Rússia

Olga Sazonova

Samara State Medical University

Email: noemail@neicon.ru
ORCID ID: 0000-0002-4130-492X
Rússia

Bibliografia

  1. Mironova L.V., Kryukova N.F., Slauta I.V., Vereshchagina S.V. Fungicidal activity of disinfectants against mold fungi found in the air of health care facilities of Neryungri Central district hospital. Yakutskiy meditsinskiy zhurnal. 2009; (3): 100–2. (in Russian)
  2. Badamshina G.G., Ziatdinov V.B., Isaeva G.Sh., Stavropol’skaya L.V., Khaldeeva E.V., Glushko N.I., et al. Estimation of fungal contamination of the air environment of the multidisciplinary medical facility. Problemy meditsinskoy mikologii. 2017; 19(1): 43–5. (in Russian)
  3. Klimko N.N., Kozlova Ya.I., Khostelidi S.N., Shadrivova O.V., Borzova Yu.V., Vasil’eva N.V. The prevalence of serious and chronic fungal diseases in Russian Federation on LIFE program model. Problemy meditsinskoy mikologii. 2014; 16(1): 3–8. (in Russian)
  4. Tupikova D.S., Lyamin A.V., Kondratenko O.V. Assessment of microbial load in internroom`s air of multidisciplinary hospital. In: Materials of the 1st International Youth Forum «Profession and Health» [Materialy 1-go Mezhdunarodnogo Molodezhnogo Foruma «Professiya i zdorov’e»]. Moscow; 2016: 131–4. (in Russian)
  5. Burova S.A. Invasive mycoses in intensive-care units: literature review (report 1). Infektsii v khirurgii. 2014; 12(2): 12–6. (in Russian)
  6. Marfenina O.E. Dangerous mildew in the environment. Priroda. 2002; 11: 1–6. (in Russian)
  7. Menegueti M.G., Ferreira L.R., Silva M.F.I., Silva A.S., Bellissimo-Rodrigues F. Assessment of microbiological air quality in hemato-oncology units and its relationship with the occurrence of invasive fungal infections: an integrative review. Rev. Soc. Bras. Med. Trop. 2013; 46(4): 391–6. https://doi.org/10.1590/0037-8682-0022-2013
  8. Chacko A., Moss R.B. Manifestations of pulmonary aspergillosis in pediatrics. Curr. Opin. Pediatr. 2020; 32(3): 389–94. https://doi.org/10.1097/MOP.0000000000000898
  9. Charushina I.P., Fel’dblyum I.V., Aleksandrova G.A., Balandina S.Yu. Comparative assessment of contamination by micromycetes of the hospital environment departments of reanimation and intensive therapy of infectious and surgical hospitals. Problemy meditsinskoy mikologii. 2014; 16(3): 83–6. (in Russian)
  10. Punchenko O.E., Kosyakova K.G., Vasil’eva N.V. The study of air microbiota in a multidisciplinary hospital in St. Petersburg. Gigiena i Sanitaria (Hygiene and Sanitation, Russian journal). 2014; 93(5): 33-36. (in Russian)
  11. Ziatdinov V.B., Badamshina G.G., Isaeva G.Sh. Mycological contamination of air assessed at medical institutions. Permskiy meditsinskiy zhurnal. 2016; 33(4): 107–12. (in Russian)
  12. Badamshina G.G., Ziatdinov V.B., Bakirov A.B., Valeev A.A., Stavropol’skaya L.V., Isaeva G.Sh., et al. The necessity of establishing standards governing the concentration of yeast-like and mould fungi in hospital environment of healthcare organizations. Meditsina truda i ekologiya cheloveka. 2017; (2): 48–52. (in Russian)
  13. Nazari Z., Ghaffari J., Ghaffari N., Ahangarkani F. A review on hypersensitivity reactions to fungal aeroallergens in patients with allergic disorders in Iran. Curr. Med. Mycol. 2019; 5(1): 42–7. https://doi.org/10.18502/cmm.5.1.537
  14. Al-Ahmad M., Jusufovic E., Arifhodzic N., Rodriguez T., Nurkic J. Association of molds and metrological parameters to frequency of severe asthma exacerbation. Allergy Asthma Clin. Immunol. 2019; 15: 29. https://doi.org/10.1186/s13223-019-0323-8
  15. Aak O.V., Sobolev A.V. The role of fungi on bronchial asthma. Problemy meditsinskoy mikologii. 2011; 13(4): 12–4. (in Russian)
  16. Kryazhev D.V. The analysis of quantitative and specific structure of the mould fungi circulating in air of multipurpose health center. Meditsinskiy al’manakh. 2019; (3–4): 92–5. https://doi.org/10.21145/2499-9954-2019-3-92-95 (in Russian)
  17. Rick E.M., Woolnough K., Pashley C.H., Wardlaw A.J. Allergic fungal airway disease. J. Investig. Allergol. Clin. Immunol. 2016; 26(6): 344–54. https://doi.org/10.18176/jiaci.0122
  18. Wickes B.L., Wiederhold N.P. Molecular diagnostics in medical mycology. Nat. Commun. 2018; 9(1): 5135. https://doi.org/10.1038/s41467-018-07556-5
  19. Aak O.V., Sobolev A.V., Ermolova S.O., Odintsova T.S. Mycogenic sensitization and the degree of gravity of bronchial asthma in the Leningrad region inhabitants. Problemy meditsinskoy mikologii. 2015; 17(1): 31–4. (in Russian)
  20. Sutton D.A., Fothergill A.W., Rinaldi M.G. Guide to Clinically Significant Fungi. Baltimore: Williams & Wilkins; 1998.

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Declaração de direitos autorais © Tupikova D.S., Berezin I.I., Zhestkov A.V., Lyamin A.V., Kozlov A.V., Sazonova O.V., 2024


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