An experimental trial of bioprophylactic formula designed to minimize combined toxicity of both lead and cadmium

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

Introduction. The increase in the body resistance to the combined effects of lead and cadmium (including systemic toxicity, cardiovascular effects, and genotoxicity) by using a specific bioprotective formula (based on theoretical knowledge and experimental research) remains a pressing challenge. However, a data search has not yielded any results on either an experimental trial or a theoretical justification of the means of biological protection against a variety of adverse effects caused by Pb and Cd combination.

Material and methods. The experiment was conducted on the outbred male rats. The animals received repeated intraperitoneal injections of water solutions of lead acetate and cadmium chloride, 3 times per week for 6 weeks. After the exposition was completed, more than 50 indices of toxic exposure (including biochemical and histo-morphological ones) were estimated in all groups of the tested animals. To assess the genotoxic effect of “in vivo” there was used amplified fragment length polymorphism (AFLP) analysis. Statistical analysis was done using Student’s t-test.

Results. We found the administration of the bioprotective formula to improve the indices of general toxicity. Genotoxicity studied using AFLP analysis of blood cells DNA was shown to be mitigated. Histo-morphological indices of Pb+Cd hepato- and nephrotoxicity improved under a bioprotective complex (BPC) administration. Blood Pb and Cd decreased during BPC administration. There was a statistically reliable decrease in the mean diameter of cardiomyocytes associated with Pb+Cd administration. These changes became less apparent with the BPC administration.

Conclusion. We developed and tested a strategy to mitigate the toxic effects of Pb and Cd at organ and organ system levels, including general toxicity, target organ toxicity (with cardiotoxicity) and genоtoxicity.

Негізгі сөздер

Авторлар туралы

Larisa Privalova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Хат алмасуға жауапты Автор.
Email: privalovali@yahoo.com
ORCID iD: 0000-0002-1442-6737

MD, Ph.D., DSci, Head of Laboratory of Scientific Basis for Biological Prophylaxis, Department of the Toxicology and Bioprophylaxis, Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Yekaterinburg, 620014, Russian Federation.

e-mail: privalovali@yahoo.com

Ресей

S. Klinova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0002-0927-4062
Ресей

I. Minigalieva

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0002-0097-7845
Ресей

Iu. Ryabova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0003-2677-0479
Ресей

M. Sutunkova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0002-1743-7642
Ресей

O. Makeev

Central Research Laboratory of the Ural Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0001-6819-3185
Ресей

I. Valamina

Central Research Laboratory of the Ural Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0001-7387-5287
Ресей

T. Bushueva

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0002-5872-2001
Ресей

S. Solovyeva

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0001-8580-403X
Ресей

V. Gurvich

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0002-6475-7753
Ресей

B. Katsnelson

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
ORCID iD: 0000-0001-8750-9624
Ресей

Әдебиет тізімі

  1. Privalova L.I., Katsnelson B.A., Minigalieva I.A., Sutunkova M.P., Makeev O.G., Valamina I.E. et al. Biological prophylaxis in the system of the management of occupational risk due to exposure of metal-containing nanoparticles. Gigiena i sanitariya [Hygiene and Sanitation, Russian journal]. 2017; 96 (12): 1187–91. https://doi.org/10.18821/00169900-2017-96-12-1187-1191 (in Russian)
  2. Katsnelson B.A., Privalova L.I., Sutunkova M.P., Minigalieva I.A., Gurvich V.B., Shur V.Ya. et al. Experimental research into metallic and metal oxide nanoparticle toxicity in vivo. In: Bioactivity of Engineered Nanoparticles. Yan B., Zhou H., Gardea-Torresdey J., eds. Springer; 2017: 259–31.
  3. Kireyeva Ye.P., Katsnelson B.A., Degtyaryova T.D., Privalova L.I., Valamina I.Ye., Beresneva O.Yu. et al. Nephrotoxic effect posed by lead and cadmium and its inhibition by a complex of bioprotective agen. Toxicol Rev. 2006; 3: 26–31. (in Russian)
  4. Katsnelson B.A., Privalova L.I., Kuzmin S.V., Kiryeva Ye.P., Krushcheva N.A., Beikin Ya.B. et al. Links of preclinical renal lesion in children at preschool age to the cadmium and lead concentration in urine. Toxicol Rev. 2006, 4: 35–41. (in Russian)
  5. Katznelson B.A., Degtyareva T.D., Privalova L.I. The principles of environmental prevention of occupational and environmentally related pathology from exposure to inorganic substances [Printsipy ekologicheskoy profilaktiki professional’noy i ekologicheski obuslovlennoy patologii ot vozdeystviya neorganicheskikh veshchestv]. Ekaterinburg: MNTsP and OZRP; 1999. 107 p. (in Russian)
  6. Degtyareva T.D., Katsnelson B.A., Privalova L.I. et al. Evaluation of the effectiveness of biological prophylaxis of lead intoxication (experimental research). Meditsina truda i promyshlennaya ekologiya [Russian Journal of Occupational Health and Industrial Ecology]. 2000; 3: 40–3. (in Russian)
  7. Katznelson B.A., Degtyareva T.D., Privalova L.I., Soloboeva Yu.I., Kireeva E.P., Minigalieva I.A. et al. Biological prevention of environmentally caused health disorders: theoretical background, experimental data, performance evaluation, practical implementation. Biosfera [Biosphere]. 2010; 2 (3): 375–85. (in Russian)
  8. Kirmizis D., Chatzidimitriou D. Antiatherogenic effects of vitamin E: the search for the Holy Grail. Vasc Health Risk Manag. 2009; 5: 767–74.
  9. Polosyants O.B., Aleksanyan L.A. Antioxidant vitamins in the prevention and treatment of cardiovascular diseases. Russkiy meditsinskiy zhurnal [Russian Medical Journal]. 2005; 11: 780–5. (in Russian)
  10. Ilyasov I.R., Beloborodov V.L., Tyukavkina N.A. et al. The use of radical cations ABST in the evaluation of the antioxidant activity of flavanoids. Farmatsiya. 2008; 6: 15–8. (in Russian)
  11. Shen J., Wang X., Zhou D., Li T., Tang L., Gong T. Modelling cadmiuminduced cardiotoxicity using human pluripotent stem cellderived cardiomyocytes. Cell Mol Med. 2018; 22 (9): 4221–35.
  12. Tarakhovsky Yu.S., Kim Yu.A., Abdrasilov B.S., Muzafarov E.N. Flavonoids: biochemistry, biophysics, medicine [Flavonoidy: biokhimiya, biofizika, meditsina]. Pushchino: Synchrobook; 2013. 310 p. (in Russian)
  13. MR 2.3.1.2432—08. The physiological requirements for energy and nutrients for various population groups of the Russian Federation. (in Russian)
  14. Uylaş M.U., Şahin A., Şahintürk V., Alataş İ.Ö. Quercetin dose affects the fate of hepatic ischemia and reperfusion injury in rats: An experimental research. Int J Surg. 2018; 53: 117–21.
  15. Li X., Jin Q., Yao Q., Xu B., Li L., Zhang S. et al. The Flavonoid Quercetin Ameliorates Liver Inflammation and Fibrosis by Regulating Hepatic Macrophages Activation and Polarization in Mice. Front Pharmacol. 2018; 9: 72. https://doi.org/10.3389/fphar.2018.00072
  16. Suidasari S., Hasegawa T., Yanaka N., Kato N. Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats. Springerplus. 2015; 4: 280. Published online 2015 Jun 19. https://doi.org/10.1186/s40064-015-1074-8
  17. Gromova O.A., Torshin I.Y., Nazarenko A.G., Kalachev A.G. Deficiency of Magnesium and Pyridoxine as a Risk Factors for Coronary Heart Disease. Kardiologiya. 2016; 56 (10): 55–62.
  18. Katznelson B.A., Degtyareva T.D., Privalova L.I. Development of tools that increase the body’s resistance to the action of inorganic pollutants of the production and the environment. Rossiyskiy khimicheskiy zhurnal. 2004; XLVIII (2): 65–72. (in Russian)
  19. Gaertner R.R., Hollebone B.R. The in vitro inhibition of hepatic ferrochelatase by divalent lead and other soft metal ions. Can J Biochem Cell Biol. 1983; 61 (4): 214–22.
  20. Rodionova L.V. Physiological role of macro- and microelements. Byulleten’ VSNTs SO RAMN [Bulletin of the VSSC SB RAMS]. 2005; 6 (44): 195–9.
  21. Gromova O.A., Egorova E.Yu., Torshin I.Yu., Gromov A.N., Gogoleva I.V. On the role of magnesium in sports medicine. Kardiologiya. 2016, 1: 1–11. (in Russian)
  22. Brenda C.T. Kieboom, Maartje N. Niemeijer, Maarten J.G. Leening, Marten E. van den Berg, Oscar H. Franco, Jaap W. Deckers et al. Serum Magnesium and the Risk of Death From Coronary Heart Disease and Sudden Cardiac Death. J Am Heart Assoc. 2016; 5 (1). https://doi.org/10.1161/JAHA.115.002707
  23. Chao S.H., Suzuki Y., Zysk J.R., Cheung W.Y. Activation of calmodulin by various metal cations as a function of ionic radius. Mol Pharmacol. 1984; 26 (1): 75–82.
  24. Suzuki Y., Chao S.H., Zysk J.H., Cheung W.Y. Stimulation of calmodulin by cadmium ion. Arch Toxicol. 1985; 57: 205–11.
  25. Paul L. Diet, nutrition and telomere length. J Nutr Biochem. 2011; 22: 895–901.
  26. Arsenic: Hygienic criteria for environmental conditions. Geneva: WHO; 1985. 185 p.
  27. Gromova O.A. Magnesia therapy in outpatient practice. Spravochnik poliklinicheskogo vracha. 2006; 1: 19–24 (in Russian)
  28. Wang P., Fan F., Li X., Sun X., Ma L., Wu J. et al. Riboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial infarction. J Mol Cell Cardiol. 2018; 115: 115–29. https://doi.org/10.1016/j.yjmcc.2018.01.006
  29. Trahtenberg I.M., Lubjanova I.P., Apyhtina E.L. The role of lead and iron as technogenic chemical pollutants in the pathogenesis of cardiovascular diseases. Therapia. 2010; 07–08 (49): 36–9. (in Russian)
  30. Chen C., Zhang S., Liu Z., Tian Y. et al. Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci Rep. 2015; 35 (3): e00214.

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© Privalova L.I., Klinova S.V., Minigalieva I.A., Ryabova I.V., Sutunkova M.P., Makeev O.G., Valamina I.E., Bushueva T.V., Solovyeva S.N., Gurvich V.B., Katsnelson B.A., 2024


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