Effect of the rs1800795 polymorphism of the IL-6 gene on echocardiographic parameters in miners with occupational dust lung pathology

Introduction. The Kuzbass coal industry is characterized by a high prevalence of occupational and industrial-related diseases. The combined effects of a complex of harmful industrial factors contribute to the formation of associated pathology of the respiratory and cardiovascular systems. The pathogenetic role of inflammation triggered by the cytokine cascade in the initiation of cardiovascular pathology has been proven. The key role in the development of which is given to cytokines with proinflammatory activity, in particular interleukin-6.Purpose of the investigation was to identify the relationship of the polymorphic locus rs1800795 of the IL-6 gene with echocardiographic parameters in miners with occupational lung dust pathology.Materials and methods. A total of one hundred fifty miners with long-term labour experience were examined. The main group consisted of 110 miners with a previously proven diagnosis of “dust lung pathology”. Then, all patients with an occupational diagnosis were divided into 3 subgroups depending on the carriage of genotypes of the rs1800795 polymorphism of the IL-6 gene. The comparison group included 40 miners working in similar conditions who do not have a proven occupational disease of the respiratory system. All patients underwent genetic studies: genotyping of single-nucleotide polymorphic loci of the rs1800795 gene of the IL-6 gene by the method of allele-specific polymerase chain reaction in real time. The morphological and functional state of the heart was assessed using echocardiography according to the standard technique on the Vivid E9 ultrasound system manufactured by General Electric.Results. Carriers of different genotypes of the polymorphic marker C(-174)G of the IL-6 gene had significantly different early filling rate and left ventricular ejection fraction. The minimum values of both echocardiographic parameters were found in the group of miners with dust lung pathology, who were owners of the heterozygous GG genotype of the C(-174)G polymorphic marker of the IL-6 gene. Differences in the structural and functional parameters of the heart were revealed between miners with dust lung pathology with different genotypes of the rs1800795 polymorphism of the IL-6 gene and patients of the comparison group. The holders of all three genotypes showed a significant increase in the average pressure in the pulmonary artery compared with the group of individuals without occupational pathology. The rate of early filling of the left ventricle, its ejection fraction, as well as the ratio of the rate of early and late filling of the left ventricle were maximum in the comparison group, having statistically significant differences with homozygotes for the G-allele among the miners with occupational lung pathology.Limitations. The study was limited by the number of miners undergoing in-depth periodic medical examination at the Clinic of the Research Institute for Complex Problems of Hygiene and Occupational Diseases. Conclusion. The results of the study indicate a predisposition of miners with occupational pathology of the bronchopulmonary system, owning the G/G genotype of the rs1800795 polymorphism of the IL-6 gene to the formation of left ventricular dysfunction. Studying the genetic predisposition to the development of structural and functional disorders of the heart among certain occupational groups will allow timely identifying workers with a high risk of chronic heart failure. The revealing initial myocardial changes based on genetic analysis will facilitate timely prevention aimed at reducing the incidence of possible cardiovascular complications against the background of occupational pathology of the respiratory system in coal mine workers.Compliance with ethical standards. The study was approved by the local Ethics Committee of the Research Institute for Complex Problems of Hygiene and Occupational Diseases (protocol of the meeting No. 4 dated November 18, 2021) conducted in accordance with the generally accepted scientific principles of the Helsinki Declaration of the World Medical Association as amended 2013. All participants gave informed voluntary written consent to participate in the study.Contribution: Kazitskaya A.S. – concept and design of the study, statistical processing, writing the text; Korotenko O.Yu. – collection of material, editing; Yadykina T.K. – collection of material and data processing.  All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.Conflict of interest. The authors declare no conflict of interest. Acknowledgment. The study had no sponsorship.Received: January 22, 2025 / Revised: February 24, 2025 /Accepted: March 26, 2025 / Published: April 30, 2025

occupational dust pathology of the lungs, cardiovascular diseases, interleukin-6, inflammation, genetic polymorphism