The use of fluorescence time-resolved microscopy to increase the endoplasmic reticulum selectivity of arylidene-imidazolones fluorogenic dyes

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Using fluorescence time-resolved microscopy (FLIM), a number of previously synthesized fluorogenic aryliden-imidazolone analogues, predominantly staining the endoplasmic reticulum (ER) of living cells, were studied. It has been shown that the use of this type of fluorescence microscopy can increase the selectivity of ER staining.

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A. Gilvanov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

编辑信件的主要联系方式.
Email: aidar_gilvanov@mail.ru
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997

A. Smirnov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Email: aidar_gilvanov@mail.ru
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997

S. Krasnova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Email: aidar_gilvanov@mail.ru
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997

I. Solovyev

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: aidar_gilvanov@mail.ru
俄罗斯联邦, Leninskiy prosp. 33/2, 119071 Moscow

A. Savitsky

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: aidar_gilvanov@mail.ru
俄罗斯联邦, Leninskiy prosp. 33/2, 119071 Moscow

Yu. Bogdanova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Email: aidar_gilvanov@mail.ru
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997

M. Baranov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Email: aidar_gilvanov@mail.ru
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997; ul. Ostrovitianova 1, Moscow, 117997

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3. Fig. 1. Micrographs of living HeLa Kyoto cells stained with dyes (I) (a), (II) (b), (III) (c), and (IV) (d), obtained using time-resolved fluorescence microscopy. Color coding reflects the fluorescence lifetimes of the dyes depending on their environment. The corresponding range of lifetimes in nanoseconds is indicated under each micrograph. For dyes with two spectral components ((I), (III), and (IV)) the amplitude-weighted average lifetime was used as the fluorescence lifetime value. Scale bar is 5 μm.

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4. Fig. 2. Micrographs of living HeLa Kyoto cells stained with dyes (I) and (III) before (a, c, respectively) and after (b, d) removal of image areas containing lipid droplets. Removal of areas containing lipid droplets was performed based on differences in the fluorescence lifetimes of substances when stained with EPR and lipid droplets. Scale bar is 5 μm.

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5. Scheme 1. Structures of dyes of the arylidene-imidazolone series studied in the work.

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