Nano-frfast: design of a new genetically-encoded far-red fluorescent label

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

We propose a new fluorogen-activating protein nano-frFAST with only 98 amino acids based on a combination of the previously created fluorogen-activating proteins nanoFAST and frFAST. A series of fluorogens with an increased system of conjugated bonds, which are potentially capable of binding to this protein, were synthesized. Based on the results of the study, a promising fluorogen which is (Z)-5-((E)-3-(4-hydroxy-2,5-dimethoxyphenyl)allylidene)-2-thioxothiazolidin-4-one (HPAR-DOM) was identified. We demonstrate that the nano-frFAST–HPAR-DOM complex can be used as a genetically encoded far-red fluorescent label for staining individual structures of living cells.

全文:

受限制的访问

作者简介

E. Sokolinskaya

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

编辑信件的主要联系方式.
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow

Yu. Bogdanova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow

I. Myasnyanko

Pirogov Russian National Research Medical University

Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow

A. Sokolov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow

S. Krasnova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow

M. Baranov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow

参考

  1. Snapp E.L. // Trends Cell Biol. 2009. V. 19. P. 649–655. https://doi.org/10.1016/j.tcb.2009.08.002
  2. Chudakov D.M., Matz M.V., Lukyanov S., Lukyanov K.A. // Physiol. Rev. 2010. V. 90. P. 1103–1163. https://doi.org/10.1152/physrev.00038.2009
  3. Hori Y., Ueno H., Mizukami S., Kikuchi K. // J. Am. Chem. Soc. 2009. V. 131. P. 16610–16611. https://doi.org/10.1021/ja904800k
  4. Mineev K.S., Goncharuk S.A., Goncharuk M.V., Povarova N.V., Sokolov A.I., Baleeva N.S., Smirnov A.Yu., Myasnyanko I.N., Ruchkin D.A., Bukhdruker S., Remeeva A., Mishin A., Borshchevskiy V., Gordeliy V., Arseniev A.S., Gorbachev D.A., Gavrikov A.S., Mishin A.S., Baranov M.S. // Chem. Sci. 2021. V. 12. P. 6719–6725. https://doi.org/10.1039/d1sc01454d
  5. Plamont M.-A., Billon-Denis E., Maurin S., Gauron C., Pimenta F.M., Specht C.G., Shi J., Quérard J., Pan B., Rossignol J., Moncoq K., Morellet N., Volovitch M., Lescop E., Chen Y., Triller A., Vriz S., Saux T. Le, Jullien L., Gautier A. // Proc. Natl. Acad. Sci. USA. 2016. V. 113. P. 497–502. https://doi.org/10.1073/pnas.1513094113
  6. Hocq R., Bottone S., Gautier A., Pflügl S. // Front. Bioeng. Biotechnol. 2023. V. 11. P. 1226889. https://doi.org/10.3389/fbioe.2023.1226889
  7. Tsien R.Y. // Annu. Rev. Biochem. 1998. V. 67. P. 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509
  8. Remington S.J. // Curr. Opin. Struct. Biol. 2006. V. 16. P. 714–721. https://doi.org/10.1016/j.sbi.2006.10.001
  9. Benaissa H., Ounoughi K., Aujard I., Fischer E., Goïame R., Nguyen J., Tebo A.G., Li C., Le Saux T., Bertolin G., Tramier M., Danglot L., Pietrancosta N., Morin X., Jullien L., Gautier A. // Nat. Commun. 2021. V. 12. P. 6989. https://doi.org/10.1038/s41467-021-27334-0
  10. Myasnyanko I.N., Gavrikov A.S., Zaitseva S.O., Smirnov A.Yu., Zaitseva E.R., Sokolov A.I., Malyshevskaya K.K., Baleeva N.S., Mishin A.S., Baranov M.S. // Chemistry. 2021. V. 27. P. 3986–3990. https://doi.org/10.1002/chem.202004760
  11. Povarova N.V., Zaitseva S.O., Baleeva N.S., Smirnov A.Yu., Myasnyanko I.N., Zagudaylova M.B., Bozhanova N.G., Gorbachev D.A., Malyshevskaya K.K., Gavrikov A.S., Mishin A.S., Baranov M.S. // Chemistry. 2019. V. 25. P. 9592–9596. https://doi.org/10.1002/chem.201901151
  12. Chen C., Tachibana S.R., Baleeva N.S., Myasnyanko I.N., Bogdanov A.M., Gavrikov A.S., Mishin A.S., Malyshevskaya K.K., Baranov M.S., Fang C. // Chemistry. 2021. V. 27. P. 8946–8950. https://doi.org/10.1002/chem.202101250
  13. Li C., Tebo A.G., Thauvin M., Plamont M.-A., Volovitch M., Morin X., Vriz S., Gautier A. // Angew. Chem. Int. Ed. Engl. 2020. V. 59. P. 17917–17923. https://doi.org/10.1002/anie.202006576
  14. Baleeva N.S., Bogdanova Y.A., Goncharuk M.V., Sokolov A.I., Myasnyanko I.N., Kublitski V.S., Smirnov A.Yu., Gilvanov A.R., Goncharuk S.A., Mineev K.S., Baranov M.S. // Int. J. Mol. Sci. 2024. V. 25. Р. 3054. https://doi.org/10.3390/ijms25053054
  15. Perfilov M.M., Zaitseva E.R., Smirnov A.Yu., Mikhaylov A.A., Baleeva N.S., Myasnyanko I.N., Mishin A.S., Baranov M.S. // Dyes Pigm. 2022. V. 198. P. 110033. https://doi.org/10.1016/j.dyepig.2021.110033
  16. Campaigne E., White R.L. // J. Heterocycl. Chem. 1988. V. 25. P. 367–373. https://doi.org/10.1002/jhet.5570250203
  17. Radi M., Botta L., Casaluce G., Bernardini M., Botta M. // J. Comb. Chem. 2010. V. 12. P. 200–205. https://doi.org/10.1021/cc9001789
  18. Voliani V., Bizzarri R., Nifosì R., Abbruzzetti S., Grandi E., Viappiani C., Beltram F. // J. Phys. Chem. B. 2008. V. 112. P. 10714–10722. https://doi.org/10.1021/jp802419h
  19. Würth C., Grabolle M., Pauli J., Spieles M., ReschGenger U. // Nat. Protoc. 2013. V. 8. P. 1535–1550. https://doi.org/10.1038/nprot.2013.087

补充文件

附件文件
动作
1. JATS XML
2. Additional materials
下载 (2MB)
3. Fig. 1. Absorption and emission spectra of the nano-frFAST–HPAR-DOM complex in phosphate buffer.

下载 (188KB)
4. Fig. 2. Representative photomicrographs of Hela Kyoto cells expressing the H2B-nano-frFAST (upper panel) and hCytokeratin-nano-frFAST (lower panel) genetic constructs in the presence of 10 μM HPAR-DOM fluorogen. Scale bar is 10 μm.

下载 (207KB)
5. Scheme 1. Structural formulas of known fluorogens of the FAST and frFAST proteins, a new proposed fluorogen for the developed nano-frFAST protein, as well as approaches to synthesis.

下载 (313KB)

版权所有 © Russian Academy of Sciences, 2025