Changes in the conductivity of bilayer lipid membranes under the action of pluronics L61 and F68: Similarities and differences

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Abstract

The effect of pluronics L61 and F68 with the same length of hydrophobic poly(propylene oxide) blocks and different lengths of hydrophilic poly(ethylene oxide) blocks on the conductivity of planar bilayer lipid membranes made of azolectin was investigated. The integral conductivity of the membranes increases with increasing concentrations of both pluronics. With the same concentration of pluronics in solution, the conductivity for L61 is higher. According to the literature data [24]. At close concentrations of membrane-bound pluronics, membrane conductivities are also close. It was concluded that the appearance of identical hydrophobic parts of pluronics L61 and F68 in the membrane causes the same increase in conductivity in the first approximation. The shape of the conductivity-concentration curves is superlinear for L61 and sublinear for F68. In the presence of both pluronics, conduction spikes with an amplitude from 10 to 300 pSm and higher are observed for approximately 40% of the membranes. We associate the observed surges in conductivity with the appearance of conductive pores or defects in the membrane. The number of pores registered in the membrane was a random variable with a large variance and did not correlate with the concentration of pluronic. The difference between the average pore conductivities for membranes with L61 and F68 was not statistically significant.

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A. A. Anosov

I. M. Sechenov First Moscow State Medical University (Sechenov University); Kotelnikov Institute of Radioengineering and Electronics of Russian Academy of Sciences

Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 119991; Moscow, 125009

E. D. Borisova

aI. M. Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 119991

O. O. Konstantinov

aI. M. Sechenov First Moscow State Medical University (Sechenov University)

Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 119991

E. Yu. Smirnova

aI. M. Sechenov First Moscow State Medical University (Sechenov University)

Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 119991

E. A. Korepanova

Pirogov Russian National Research Medical University

Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 117997

V. A. Kazamanov

MIREA – Russian Technological University

Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 119991

A. S. Derunets

National Research Centre “Kurchatov Institute”

Email: ryleeva_e_d@staff.sechenov.ru
Russian Federation, Moscow, 123182

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3. Fig. 1. Experimental dependences of the specific conductivity of azolectin membranes (a) on the concentration of L61 and F68 in the surrounding solution, (b) on the relative concentration of Pluronics bound to the membrane; approximating dependences of the specific conductivity g on the concentration C of Pluronics in solution: gL₆₁ = 15.3 × C¹.³ and gF₆₈ = 12.9 × C ⁰.⁷ pS/mm²; conductivity of control membranes 42 ± 6 pS/mm². The applied voltage did not exceed 50 mV. Surrounding solution: 0.1 M KCl. Standard errors are given.

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4. Fig. 2. Stepped tracks and histograms of conductivity of azolectin membranes in 0.1 M KCl: (a) control, (b) with the addition of 12 μM L61, (c) 26 μM F68. Voltage on the membrane is 25 mV.

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5. Fig. 3. Conductivity tracks and histograms of azolectin membranes in 0.1 M KCl: (a) with the addition of 8 μM L61, (b) 26 μM F68. The voltage on the membrane is 25 mV.

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6. Fig. 4. Fluctuations in the conductivity of azolectin membranes in 0.1 M KCl with the addition of 16 μM L61: (a) track, (b) histogram, (c) section of the track on an enlarged scale, (d) autocorrelation function of conductivity calculated from experimental data and approximated by an exponential. Voltage on the membrane is 25 mV.

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7. Fig. 5. Fluctuations in the conductivity of azolectin membranes in 0.1 M KCl with the addition of 26 μM F68: (a) track, (b) histogram (a local maximum in the region of 60 pS is shown, the histogram maximum is at 28 pS), (c) a section of the track on an enlarged scale, (d) autocorrelation function of conductivity calculated from experimental data and approximated by an exponential. The voltage on the membrane is 25 mV.

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