Эффекты вспомогательных репродуктивных технологий на социальное поведение мышей линии BTBR – модели расстройств аутистического спектра

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Аннотация

Работа является первым исследованием влияния такой вспомогательной репродуктивной технологии (ВРТ), как культивирование in vitro преимплантационных эмбрионов на социальное поведение потомков мышей линии BTBR (BTBR T + Itpr3tf/J), которая представляет собой идиопатическую модель аутизма. В качестве контроля были использованы мыши C57BL/6J. У взрослых потомков мышей, полученных в результате естественного спаривания (группы C57BL/6J и BTBR), либо после культивирования in vitro и переноса эмбрионов соответствующих линий мышей псевдобеременным самкам C57BL/6J (группы ВРТ-C57BL/6J и ВРТ-BTBR), было изучено социальное поведение. При межлинейном сравнении было показано, что мыши BTBR имеют сниженные уровни социального распознавания и мотивации по сравнению с мышами C57BL/6J. У самок ВРТ-C57BL/6J и ВРТ-BTBR было установлено снижение интереса к новому партнеру в сессии на социальное распознавание по сравнению с естественно зачатыми животными того же пола. Результаты поведенческого теста свидетельствуют о влиянии культивирования in vitro на социальное распознавание у потомков мышей линий C57BL/6J и BTBR, но только у самок.

Об авторах

И. Н. Рожкова

Институт цитологии и генетики Сибирского отделения Российской академии наук

Email: amstis@yandex.ru
Россия, Новосибирск

В. А. Напримеров

Институт цитологии и генетики Сибирского отделения Российской академии наук

Email: amstis@yandex.ru
Россия, Новосибирск

В. С. Козенева

Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университет

Email: amstis@yandex.ru
Россия, Новосибирск; Россия, Новосибирск

Т. А. Яцук

Институт цитологии и генетики Сибирского отделения Российской академии наук

Email: amstis@yandex.ru
Россия, Новосибирск

Д. А. Лебедева

Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университет

Email: amstis@yandex.ru
Россия, Новосибирск; Россия, Новосибирск

Т. А. Рахманова

Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университет

Email: amstis@yandex.ru
Россия, Новосибирск; Россия, Новосибирск

Т. Н. Игонина

Институт цитологии и генетики Сибирского отделения Российской академии наук

Email: amstis@yandex.ru
Россия, Новосибирск

Е. Ю. Брусенцев

Институт цитологии и генетики Сибирского отделения Российской академии наук

Email: amstis@yandex.ru
Россия, Новосибирск

С. В. Окотруб

Институт цитологии и генетики Сибирского отделения Российской академии наук

Email: amstis@yandex.ru
Россия, Новосибирск

С. Я. Амстиславский

Институт цитологии и генетики Сибирского отделения Российской академии наук

Автор, ответственный за переписку.
Email: amstis@yandex.ru
Россия, Новосибирск

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© И.Н. Рожкова, С.В. Окотруб, Е.Ю. Брусенцев, Т.Н. Игонина, Т.А. Рахманова, Д.А. Лебедева, Т.А. Яцук, В.С. Козенева, В.А. Напримеров, С.Я. Амстиславский, 2023