2-APB prevents atrophic changes and alters cellular signalling during unloading of rat M. Soleus

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IP3 receptors are found in significant quantities in muscle fibers in the sarcoplasmic reticulum, nucleus and mitochondria. We hypothesized that activation of IP3 receptors (IP3Rs) during muscle unloading may induce a weak calcium release signal, both cytosolic and nucleoplasmic, that promotes (possibly with other signaling cascades) the activation of transcription factors, leading to the expression or repression of genes involved in muscle phenotype. This hypothesis was tested by blocking IP3R during unloading of rat muscles by administering 2-APB (2-aminoethoxydiphenyl borate). Wistar rats were administered intraperitoneally at a dose of 10 mg/mg in 5 % DMSO daily. We found that the IP3R state influences the development of atrophic processes in the postural m. soleus during unloading. Administration of the IP3R blocker 2-APB to animals successfully prevented a decrease in m. soleus cross-sectional area (CSA) of both fast and slow muscle fibers. The slowdown in CSA decrease upon administration IP3R inhibitor during 7 days m. soleus unloading is associated with the prevention of a decrease in ribosomal biogenesis and an increase in the expression of autophagy markers ULK-1 and IL-6.

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作者简介

K. Zaripova

Institute of Biomedical Problems RAS

Email: Nemirovskaya@bk.ru
俄罗斯联邦, Moscow

R. Bokov

Institute of Biomedical Problems RAS

Email: Nemirovskaya@bk.ru
俄罗斯联邦, Moscow

K. Sharlo

Institute of Biomedical Problems RAS

Email: Nemirovskaya@bk.ru
俄罗斯联邦, Moscow

S. Belova

Institute of Biomedical Problems RAS

Email: Nemirovskaya@bk.ru
俄罗斯联邦, Moscow

T. Nemirovskaya

Institute of Biomedical Problems RAS

编辑信件的主要联系方式.
Email: Nemirovskaya@bk.ru
俄罗斯联邦, Moscow

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2. Fig. 1. Animal body weight (a), m. soleus weight (b) and m. soleus wet weight index (c). 7C – vivaro control with placebo administration, 7CA – vivaro control with 2-APB administration, 7HS – 7-day suspension with placebo administration, 7HSA – 7-day suspension with 2-APB administration. * – significant differences between groups (p < 0.001).

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3. Fig. 2. Cross-sectional area of ​​slow fibers (a), fast fibers (b) and micrographs of immunohistochemical staining of slow myosin (green) and fast myosin (red) (c). 7C – vivaro control with placebo administration, 7CA – vivaro control with 2-APB administration, 7HS – 7-day suspension with placebo administration, 7HSA – 7-day suspension with 2-APB administration. * – significant differences between groups. The size bar is 200 μm.

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4. Fig. 3. Content of 18S rRNA (a), 28S rRNA (b) in m. soleus after administration of 2-APB against the background of 7-day suspension. 7C – vivaro control with administration of placebo, 7CA – vivaro control with administration of 2-APB, 7HS – 7-day suspension with administration of placebo, 7HSA – 7-day suspension with administration of 2-APB, * – significant differences between groups.

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5. Fig. 4. Expression of MuRF1 (a), MAFbx (b), and ubiquitin (c) mRNA in m. soleus upon administration of 2-APB during 7-day suspension. 7C – vivaro control with administration of placebo, 7CA – vivaro control with administration of 2-APB, 7HS – 7-day suspension with administration of placebo, 7HSA – 7-day suspension with administration of 2-APB. * – significant differences between groups.

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6. Fig. 5. Expression of ULK1 (a) and IL6 (b) mRNA in m. soleus upon administration of 2-APB against the background of 7-day suspension. 7C – vivaro control with administration of placebo, 7CA – vivaro control with administration of 2-APB, 7HS – 7-day suspension with administration of placebo, 7HSA – 7-day suspension with administration of 2-APB. * – significant differences between groups.

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7. Fig. 6. CaMKIIb phosphorylation level in m. soleus after 2-APB administration against the background of 7-day suspension. 7C – vivaro control with placebo administration, 7CA – vivaro control with 2-APB administration, 7HS – 7-day suspension with placebo administration, 7HSA – 7-day suspension with 2-APB administration. * – significant differences between groups.

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8. Fig. 7. Expression of IP3R mRNA in m. soleus upon administration of 2-APB against the background of 7-day suspension. 7C – vivaro control with administration of placebo, 7CA – vivaro control with administration of 2-APB, 7HS – 7-day suspension with administration of placebo, 7HSA – 7-day suspension with administration of 2-APB. * – significant differences between groups.

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