Electrical Activity of the Uterus in Rats at Different Stages of the Estrous Cycle

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

Experiments were performed under urethane anesthesia on sexually mature non-pregnant female rats (n = 36). Pattern of electrical activity of the uterus in different phases of the estrous cycle, determined by the vaginal cytology (staining by Romanovsky dye), was studied. Electrohysterograms from the middle third of the left horn of the uterus were recorded using pressure bipolar electrodes. Increased excitability and conductivity of the uterus smooth myocytes occur during the stages of proestrus, estrus and metestrus, and their suppression is observed in diestrus. The maximum amplitude of motor potentials, compared to diestrus, was detected at the pro- (174 ± 17 μV) and metestrus (202 ± 20 μV) stages, while their mean frequency was high at the metestrus [4.4 (4.0; 5.0) Hz] and estrus [4.0 (4.0; 5.0) Hz)], exceeding those indicators for diestrus (86 ± 9 μV and 1.9 (1.3; 3.2) Hz approximately twice. Burst phase duration [40 (32; 50) and 36 (28; 47) s] of uterus periodic electrical activity had it maximum while rest period had it minimum [46 (40; 55) and 42 (26; 84) s] at proestrus and estrus stages which is also reflected in the high values of duty cycle – 0.46 (0.43; 0.50) and 0.45 (0.26; 0.63), respectively. Similar indicators for the diestrus stage were 27 (22; 32) and 80 (58; 124) s, 0.27 (0.18; 0.34). It is assumed that the highest excitability of the myometrium and its rhythmogenic areas during the metestrus stage, an increase in myometrium’s excitation conduction rate during proestrus and estrus, are due to an increase of electrical coupling between uterus smooth muscle cells.

全文:

受限制的访问

作者简介

S. Rutkevich

Belarusian State University; Belarusian State Medical University

编辑信件的主要联系方式.
Email: rutkevitch@inbox.ru
白俄罗斯, Minsk; Minsk

P. Pihul

Belarusian State University

Email: rutkevitch@inbox.ru
白俄罗斯, Minsk

Yu. Panimatska

Belarusian State University

Email: rutkevitch@inbox.ru
白俄罗斯, Minsk

V. Kazakevich

Belarusian State University; Belarusian State Medical University

Email: rutkevitch@inbox.ru
白俄罗斯, Minsk; Minsk

I. Veres

Belarusian State Medical University

Email: rutkevitch@inbox.ru
白俄罗斯, Minsk

A. Sidorov

Belarusian State University

Email: rutkevitch@inbox.ru
白俄罗斯, Minsk

A. Chumak

Belarusian State University

Email: rutkevitch@inbox.ru
白俄罗斯, Minsk

参考

  1. Wray S, Prendergast C (2019) The myometrium: from excitation to contractions and labour. Adv Exp Med Biol 1124: 233–263. https://doi.org/10.1007/978-981-13-5895-1_10
  2. Aguilar HN, Mitchell BF (2010) Physiological pathways and molecular mechanisms regulating uterine contractility. Hum Reprod Update 16: 725–744. https://doi.org/10.1093/humupd/dmq016
  3. Wray S, Arrowsmith S (2021) Uterine excitability and ion channels and their changes with gestation and hormonal environment. Annu Rev Physiol 83: 331–357. https://doi.org/10.1146/annurev-physiol-032420-035509
  4. Черток ВM, Храмова ИА, Коцюба АЕ (2020) Газотрансмиттеры в регуляции функций внутриорганных кровеносных сосудов матки. Морфология 157: 98–111 [Chertok VM, Khramova IA, Kotsyuba AE (2020) Gasotransmitters in the regulation of the functions of the intraorganic blood vessels of the uterus. Morphology 157: 98–111. (In Russ)]. https://doi.org/10.34922/AE.2020.157.1.015
  5. Arrowsmith S, Kendrick A, Hanley JA, Noble K, Wray S (2014) Myometrial physiology – time to translate? Exp Physiol 99: 495–502. https://doi.org/10.1113/expphysiol.2013.076216
  6. Garrett AS, Roesler MW, Athavale ON, Du P, Means SA, Clark AR, Cheng LK (2024) Multichannel mapping of in vivo rat uterine myometrium exhibits both high and low frequency electrical activity in non-pregnancy. Sci Rep 14: 7316. https://doi.org/10.1038/s41598-024-57734-3
  7. Hutchings G, Williams O, Cretoiu D, Ciontea SM (2009) Myometrial interstitial cells and the coordination of myometrial contractility. J Cell Mol Med 13: 4268–4282. https://doi.org/10.1111/j.1582-4934.2009.00894
  8. Smith CC, Cizza G, Gomez M, Greibler C, Gold PW, Sternberg EM (1994) The estrous cycle and pituitary-ovarian function in Lewis and Fischer rats. Neuroimmunomodulation 1: 231–235. https://doi.org/10.1159/000097170
  9. Cora MC, Kooistra L, Travlos G (2015) Vaginal Cytology of the Laboratory Rat and Mouse: Review and Criteria for the Staging of the Estrous Cycle Using Stained Vaginal Smears. Toxicol Pathol 43: 776–793. https://doi.org/10.1177/0192623315570339
  10. Goldman JM, Murr AS, Cooper RL (2007) The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies. Birth Defects Res B Dev Reprod Toxicol 80: 84–97. https://doi.org/10.1002/bdrb.20106
  11. Wray S, Jones K, Kupittayanant S, Li Y, Matthew A, Monir-Bishty E, Noble K, Pierce SJ, Quenby S, Shmygol AV (2003) Calcium signaling and uterine contractility. J Soc Gynecol Invest 10: 252–264. https://doi.org/10.1016/s1071-5576(03)00089-3
  12. Malik M, Roh M, England SK (2021) Uterine contractions in rodent models and humans. Acta Physiol 231: e13607. https://doi.org/10.1111/apha.13607
  13. Карева ЕН, Булгакова ВА, Гуторова ДС, Олейникова ОМ, Кононова ИН, Горбунов АА, Бреусенко ВГ, Фисенко ВП (2020) Мембранный рецептор прогестерона PGRMC1 – потенциальная мишень лекарственных средств. Экспер клин фармакол 83(6): 19–29 [Kareva EN, Bulgakova VA, Gutorova DS, Olejnikova OM, Kononova IN, Gorbunov AA, Breusenko VG, Fisenko VP (2020) The membrane-bound progesterone receptor PGRMC1 is a potential drug target. Exp Clin Pharmacol 83(6): 19–29. (In Russ)]. https://doi.org/10.30906/0869-2092-2020-83-6-19-29
  14. Кузьминых ТУ, Борисова ВЮ, Николаенков ИП, Козонов ГР, Толибова ГХ (2019) Роль биологически активных молекул в развитии сократительной деятельности матки. Журн акушер и женск болезн 68: 21–27 [Kuz'minyh TU, Borisova VYu, Nikolaenkov IP, Kozonov GR, Tolibova GH (2019) The role of biologically active molecules in the development of uterine contractile activity]. J Obstetrics Female Diseas 68: 21–27. (In Russ)]. https://doi.org/10.17816/JOWD68121-27
  15. Arthur P, Taggart MJ, Mitchell BF (2007) Oxytocin and parturition: A role for increased myometrial calcium and calcium sensitization? Front Biosci 12: 619–633. https://doi.org/10.2741/2087
  16. Domino M, Pawlinski B, Gajewski Z (2017) Biomathematical pattern of EMG signal propagation in smooth muscle of the non-pregnant porcine uterus. PLoS One 12: e0173452. https://doi.org/10.1371/journal.pone.0173452.
  17. Zhang Y, Qian J, Zaltzhendler O, Bshara M, Jaffa AJ, Grisaru D, Duan E, Elad D (2019) Analysis of in vivo uterine peristalsis in the non-pregnant female mouse. Interface Focus 9: 20180082. https://doi.org/10.1098/rsfs.2018.0082
  18. Domino M, Pawlinski B, Gajewska M, Jasinski T, Sady M, Gajewski Z (2018) Uterine EMG activity in the non-pregnant sow during estrous cycle. BMC Vet Res 14: 176. https://doi.org/10.1186/s12917-018-1495-z
  19. Young RC (2007) Myocytes, myometrium, and uterine contractions. Ann N Y Acad Sci 1101: 72–84. https://doi.org/10.1196/annals.1389.038
  20. Wray S, Noble K (2008) Sex hormones and excitation-contraction coupling in the uterus: The effects of oestrous and hormones. J Neuroendocrinol 20: 451–461. https://doi.org/10.1111/j.1365-2826.2008.01665.x
  21. Толибова ГX, Константинова НН (2007) Экспериментальные исследования сократительной активности матки. Журн акушер и женск болезн 56(3): 134–143 [Tolibova GX, Konstantinova NN (2007) Experimental studies of uterine contractile activity. J Obstetrics Female Diseas 56(3): 134–143. (In Russ)].
  22. Young RC (2018) The uterine pacemaker of labor. Best Pract Res Clin Obstet Gynaecol 52: 68–87. https://doi.org/10.1016/j.bpobgyn.2018.04.002
  23. Maul H, Maner WL, Saade GR, Garfield RE (2003) The physiology of uterine contractions. Clin Perinatol 30: 665–676. https://doi.org/10.1016/s0095-5108(03)00105-2
  24. Rabotti C, Mischi M (2015) Propagation of electrical activity in uterine muscle during pregnancy: a review. Acta Physiol 213: 406–416. https://doi.org/10.1111/apha.12424
  25. Sidorov AV, Kazakevich VB, Moroz LL (1999) Nitric oxide selectively enhances cAMP levels and electrical coupling between identified RPaD2/VD1 neurons in the CNS of Lymnaea stagnalis (L.). Acta Biol Hung 50: 229–233. https://doi.org/10.1007/BF03543044
  26. Kazaryan KV, Piliposyan TA, Unanyan NG, Mkrtchyan EKh (2017) The role of the ovarian horn locus in regulation of spontaneous electric activity of myometrial rhythmogenic areas. J Evol Biochem Phys 53: 414–422. https://doi.org/10.1134/S0022093017050076
  27. Казарян КВ, Унанян НГ, Пилипосян ТА (2016) Синхронизация электрической активности ритмогенных областей миометрия при воздействии окситоцина. Рос физиол журн им ИМ Сеченова 102(3): 317–329. [Kazaryan KV, Unanyan NG, Piliposyan TA (2016) Synchronization of electrical activity of rhythmogenic areas of the myometrium under the influence of oxytocin. Russ J Physiol 102(3): 317–329. (In Russ)].
  28. Garfield RE, Ali M, Yallampalli C, Izumi H (1995) Role of gap junctions and nitric oxide in control of myometrial contractility. Semin Perinatol 19: 41–51. https://doi.org/10.1016/s0146-0005(95)80046-8
  29. Rengarajan A, Mauro AK, Boeldt DS (2020) Maternal disease and gasotransmitters. Nitric Oxide 96: 1–12. https://doi.org/10.1016/j.niox.2020.01.001
  30. Bao S, Rai J, Schreiber J (2002) Expression of nitric oxide synthase isoforms in human pregnant myometrium at term. J Soc Gynecol Invest 9: 351–356. https://doi.org/10.1177/107155760200900605

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Cytological preparations of rat vaginal swabs at different stages of the estrous cycle: (a) - diestrus, (b) - proestrus, (c) - estrus, (d) - metestrus; 1 - leukocytes, 2 - mucus, 3 - epithelial cells with nuclei, 4 - keratinised eggless epithelial scales, 5 - bacterial contamination. Image on the left - ×10 objective, on the right - ×40 objective. The calibration line is 100 µm.

下载 (5MB)
索引源数据
3. Fig. 2. Electrical activity of the rat uterus at different stages of the estrous cycle: (a) - diestrus, (b) - proestrus, (c) - estrus, (d) - metestrus. Part (d) shows the burst and pause phases of the electrical activity period (cycle period). Calibration lines: in amplitude - 100 μV, in time - 30 s.

下载 (364KB)
索引源数据
4. Fig. 3. Phase duration of the periods of myometrial electrical activity at different stages of the estrous cycle. The value of the index (numbers above the bar) and the number of analysed periods of electrical activity (n) are indicated. Data are in the form of median ± interquartile range. The F-criterion value and significance level (p) of the Kruskal-Wallis ANOVA (one-factor analysis of variance) for the whole data series are given. The Mann-Whitney z-score and significance level (p) for statistically significant differences (*) between pairs of data are given.

下载 (227KB)
索引源数据
5. Fig. 4. Filling factors of the periods of myometrial electrical activity at different stages of the estrous cycle: DiE - diestrus, ProE - proestrus, Estrus - estrus, MetE - metestrus. Other designations are the same as in Fig. 3.

下载 (115KB)
索引源数据
6. Fig. 5. Amplitude characteristics of myometrial electrical activity at different stages of the estrous cycle: (a) - maximum amplitude, (b) - average amplitude; DiE - diestrus, ProE - proestrus, Estrus - estrus, MetE - metestrus. The value of the index (numbers above the bar) and the number of animals studied (n) are indicated. Data are in the form of mean ± error of the mean. The F-criterion and significance level (p) of one-way ANOVA for the whole data series are given. For statistically significant differences (*) between pairs of data, Student's t-criterion and significance level (p) are given.

下载 (191KB)
索引源数据
7. Fig. 6. Frequency characteristics of myometrial electrical activity at different stages of the estrous cycle: (a) - dominant frequency, (b) - average frequency; DiE - diestrus, ProE - proestrus, Estrus - estrus, MetE - metestrus. Index value (numbers above the bar) and number of animals studied (n) are indicated. Data as median ± interquartile range. The F-criterion value and significance level (p) of the Kruskal-Wallis ANOVA (one-factor analysis of variance) for the whole data series are given. The Mann-Whitney z-score and significance level (p) for statistically significant differences (*) between pairs of data are given.

下载 (207KB)
索引源数据

版权所有 © Russian Academy of Sciences, 2025