LINC00891 Promotes Tumorigenesis and Metastasis of Thyroid Cancer by Regulating SMAD2/3 via EZH2


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Abstract

Background:Thyroid cancer (TC), the most common endocrine malignant tumor, is increasingly causing a huge threat to our health nowadays.

Methods:To explore the tumorigenesis mechanism of thyroid cancer, we identified that long intergenic non-coding RNA-00891 (LINC00891) was upregulated in TC using the Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and local databases. LINC00891 expression was correlated with histological type and lymph node metastasis (LNM). The high expression of LINC00891 could serve as a diagnostic marker for TC and its LNM. In vitro experiments demonstrated that LINC00891 knockdown could inhibit cell proliferation, migration, invasion and prompt apoptosis and G1 arrest of TC cells. We also investigated the related mechanisms of LINC00891 promoting TC progression using RNA sequencing, Gene Set Enrichment Analysis, and Western blotting.

Results:Our experiments demonstrated that LINC00891 promoted TC progression via the EZH2-SMAD2/3 signaling axis. In addition, overexpression of EZH2 could reverse the suppressive epithelial-to-mesenchymal transition (EMT) caused by LINC00891 knockdown.

Conclusion:In conclusion, the LINC00891/EZH2/SMAD2/3 regulatory axis participated in tumorigenesis and metastasis of thyroid cancer, which may provide a novel target for treatment.

About the authors

Yuhao Si

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Jialiang Wen

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine

Email: info@benthamscience.net

Chunlei Hu

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Hao Chen

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityMedical University

Email: info@benthamscience.net

Lizhi Lin

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Yiying Xu

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Disuo Ren

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Xinyu Meng

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Yinghao Wang

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Erjie Xia

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Email: info@benthamscience.net

Adheesh Bhandari

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Author for correspondence.
Email: info@benthamscience.net

Ouchen Wang

Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University

Author for correspondence.
Email: info@benthamscience.net

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