Investigation of Antifungal Action of Fractions C17H31NO15 Isolated from Artemisia herba-alba extract versus Isolated Aspergillus niger from Zee maize


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Abstract

Background:Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning.

Objective:Investigation of antifungal impact of various fractions of methanol extract of Artemisia herba-alba to Aspergillus niger as a plant pathogen.

Methods:Artemisia herba-alba extract was purified using column chromatography, giving various antifungal fractions tested versus A. niger.

Results:The 6th fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02±4.9 µg/ml, which was identified using Mass spectroscopy, 1HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated A. niger was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity.

Conclusion:These results revealed the possibility of using Artemisia herba-alba methanol extract as a promising antifungal versus phytopathogenic fungi, especially A. niger after more verification of results.

About the authors

Basma Amin

Department of Microbiology, The Regional Center for Mycology and Biotechnology, Al-Azhar University

Author for correspondence.
Email: info@benthamscience.net

Nahed Ayyat

Botany and Microbiology Department, Faculty of Science, Benha University

Email: info@benthamscience.net

Reyad Mohamed El-Sharkawy

Botany and Microbiology Department, Faculty of Science, Benha University

Email: info@benthamscience.net

Asmaa Hafez

Botany and Microbiology Department, Faculty of Science, Benha University

Email: info@benthamscience.net

References

  1. Chatterjee S, Kuang Y, Splivallo R, Chatterjee P, Karlovsky P. Interactions among filamentous fungi Aspergillus niger, Fusarium verticillioides and Clonostachys rosea: Fungal biomass, diversity of secreted metabolites and fumonisin production. BMC Microbiol 2016; 16(1): 83-3. doi: 10.1186/s12866-016-0698-3 PMID: 27165654
  2. Brauer VS, Rezende CP, Pessoni AM, et al. Antifungal agents in agriculture: Friends and foes of public health. Biomolecules 2019; 9(10): 521. doi: 10.3390/biom9100521 PMID: 31547546
  3. Lee SH, Oh YT, Lee DY, Cho E, Hwang BS, Jeon J. Large-scale screening of the plant extracts for antifungal activity against the plant pathogenic fungi. Plant Pathol J 2022; 38(6): 685-91. doi: 10.5423/PPJ.NT.07.2022.0098 PMID: 36503198
  4. Samson RA, Houbraken J, Summerbell RC, Flannigan B, Miller JD. Common and important species of fungi and actinomycetes in indoor environments. Microorganisms in Home and Indoor Work Environments. CRC 2001; pp. 287-92.
  5. Ruiz-Vásquez L, Ruiz Mesia L, Ceferino HD, et al. Antifungal and herbicidal potential of piper essential oils from the peruvian amazonia. Plants 2022; 11(14): 1793. doi: 10.3390/plants11141793 PMID: 35890427
  6. Billing J, Sherman PW. Antimicrobial functions of spices: Why some like it hot. Q Rev Biol 1998; 73(1): 3-49. doi: 10.1086/420058 PMID: 9586227
  7. Tapsell LC, Hemphill I, Cobiac L, et al. Health benefits of herbs and spices: The past, the present, the future. Med J Aust 2006; 185(S4): S1-S24. doi: 10.5694/j.1326-5377.2006.tb00548.x PMID: 17022438
  8. Mehrnia M, Akaberi M, Amiri MS, Nadaf M, Emami SA. Ethnopharmacological studies of medicinal plants in central Zagros, Lorestan Province, Iran. J Ethnopharmacol 2021; 280: 114080. doi: 10.1016/j.jep.2021.114080 PMID: 33798662
  9. Ayalew H, Tewelde E, Abebe B, Alebachew Y, Tadesse S. Endemic medicinal plants of Ethiopia: Ethnomedicinal uses, biological activities and chemical constituents. J Ethnopharmacol 2022; 293: 115307. doi: 10.1016/j.jep.2022.115307 PMID: 35452775
  10. Brown DE, Walton NJ. Chemicals from plants: Perspectives on plant secondary products: World Scientific Publishing London 1991.
  11. Meskin MS. Phytochemicals in nutrition and healthCRC press: Boca Ration. 2002; p. 123. doi: 10.1201/9781420031690
  12. Price JR, Lamberton JA, Culvenor CCJ. The australian phytochemical survey: Historical aspects of the CSIRO search for new drugs in Australian plants. Hist Rec Aust Sci 1992; 9(4): 335-56. doi: 10.1071/HR9930940335
  13. Arnao MB, Cano A, Hernández-Ruiz J. Phytomelatonin: An unexpected molecule with amazing performances in plants. J Exp Bot 2022; 73(17): 5779-800. doi: 10.1093/jxb/erac009 PMID: 35029657
  14. Abu-Darwish MS, Cabral C, Gonçalves MJ, et al. Artemisia herba-alba essential oil from Buseirah (South Jordan): Chemical characterization and assessment of safe antifungal and anti-inflammatory doses. J Ethnopharmacol 2015; 174: 153-60. doi: 10.1016/j.jep.2015.08.005 PMID: 26277492
  15. Amor G, Caputo L, La Storia A, De Feo V, Mauriello G, Fechtali T. Chemical composition and antimicrobial activity of Artemisia herba-alba and origanum majorana essential oils from Morocco. Molecules 2019; 24(22): 4021. doi: 10.3390/molecules24224021 PMID: 31698834
  16. Bouafia M, Amamou F, Gherib M, Benaissa M, Azzi R, Nemmiche S. Ethnobotanical and ethnomedicinal analysis of wild medicinal plants traditionally used in Naâma, southwest Algeria. Vegetos 2021; 34(3): 654-62. doi: 10.1007/s42535-021-00229-7 PMID: 34131369
  17. Naziha M, Mervat ME, Noha MA. Endophytic fungi of some medicinal plants in Egypt. Egypt Acad J Biol Sci G Microbiol 2016; 8(1): 65-78.
  18. Göttlich E, van der Lubbe W, Lange B, et al. Fungal flora in groundwater-derived public drinking water. Int J Hyg Environ Health 2002; 205(4): 269-79. doi: 10.1078/1438-4639-00158 PMID: 12068746
  19. Jimenez L, Bosko Y, Smalls S, Ignar R, English D. Molecular detection and identification of Aspergillus niger contamination in cosmetic/pharmaceutical raw materials and finished products. J Rapid Methods Autom Microbiol 1999; 7(1): 39-46. doi: 10.1111/j.1745-4581.1999.tb00370.x
  20. Zaker M, Mosallanej H. Antifungal activity of some plant extracts on Alternaria alternata, the causal agent of alternaria leaf spot of potato. Pak J Biol Sci 2010; 13(21): 1023-9. doi: 10.3923/pjbs.2010.1023.1029 PMID: 21313872
  21. Sharma RA, Chandrawat P, Sharma S, Sharma D, Sharma B, Singh D. Ethnomedicinal, pharmacological properties and chemistry of some medicinal plants of Boraginaceae in India. J Med Plants Res 2010; 5(3): 441-4.
  22. Shittul LAJ, Bankole MA, Ahmed T, et al. Antibacterial and antifungal activities of essential oils of crude extracts of Sesame radiatum against some common pathogenic micro-organisms. Biol Sci 2007; 13: 1023-9.
  23. Pereira C, Barros L, Ferreira ICFR. Extraction, identification, fractionation and isolation of phenolic compounds in plants with hepatoprotective effects. J Sci Food Agric 2016; 96(4): 1068-84. doi: 10.1002/jsfa.7446 PMID: 26333346
  24. Abd Rashed A, Rathi DNG, Nasir ANAH, Abd Rahman AZ. Antifungal properties of essential oils and their compounds for application in skin fungal infections: Conventional and nonconventional approaches. Molecules 2021; 26(4): 1093. doi: 10.3390/molecules26041093 PMID: 33669627
  25. Yosri M, Amin BH, Abed NN, Elithy AS, Kareem SM, Sidkey NM. Identification of novel bioactive compound derived from Rheum officinalis against campylobacter jejuni NCTC11168. Sci World J 2020; 2020: 1-9. doi: 10.1155/2020/3591276 PMID: 32665768
  26. Teodori L, Tagliaferri F, Stipa F, et al. Selection, establishment and characterization of cell lines derived from a chemically-induced rat mammary heterogeneous tumor, by flow cytometry, transmission electron microscopy, and immunohistochemistry. In Vitro Cell Dev Biol Anim 2000; 36(3): 153-62. doi: 10.1290/1071-2690(2000)0362.0.CO;2 PMID: 10777054
  27. Gomha SM, Riyadh SM, Mahmmoud EA, Elaasser MM. Synthesis and anticancer activities of thiazoles, 1,3-thiazines, and thiazolidine using chitosan-grafted-poly(vinylpyridine) as basic catalyst. Heterocycles 2015; 91: 1227-43. doi: 10.3987/COM-15-13210 PMID: 26703554
  28. Ahmed HY, Kareem SM, Atef A, et al. Optimization of supercritical carbon dioxide extraction of Saussurea costus oil and its antimicrobial, antioxidant, and anticancer activities. Antioxidants 2022; 11(10): 1960. doi: 10.3390/antiox11101960 PMID: 36290683
  29. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2): 55-63. doi: 10.1016/0022-1759(83)90303-4 PMID: 6606682
  30. Vijayan P, Raghu C, Ashok G, Dhanaraj SA, Suresh B. Antiviral activity of medicinal plants of Nilgiris. Indian J Med Res 2004; 120(1): 24-9. PMID: 15299228
  31. Amin Sk A, Nilanjan A, Tarun J. Design of aminopeptidase N inhibitors as anti-cancer agents. J Med Chem 2018; 61(15): 6468-90.
  32. Li J, Gu F, Wu R, Yang J, Zhang KQ. Phylogenomic evolutionary surveys of subtilase superfamily genes in fungi. Sci Rep 2017; 7(1): 45456. doi: 10.1038/srep45456 PMID: 28358043
  33. Marín-Menguiano M, Moreno-Sánchez I, Barrales RR, Fernández-Álvarez A, Ibeas JI. N-glycosylation of the protein disulfide isomerase Pdi1 ensures full Ustilago maydis virulence. PLoS Pathog 2019; 15(11): e1007687. doi: 10.1371/journal.ppat.1007687 PMID: 31730668
  34. Alzoreky NS, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol 2003; 80(3): 223-30. doi: 10.1016/S0168-1605(02)00169-1 PMID: 12423924
  35. Castro SBR, Leal CAG, Freire FR, Carvalho DA, Oliveira DF, Figueiredo HCP. Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria. Braz J Microbiol 2008; 39(4): 756-60. doi: 10.1590/S1517-83822008000400030 PMID: 24031303
  36. Doddanna S, Patel S, Sundarrao M, Veerabhadrappa R. Antimicrobial activity of plant extracts on Candida albicans: An in vitro study. Indian J Dent Res 2013; 24(4): 401-5. doi: 10.4103/0970-9290.118358 PMID: 24047829
  37. Samadi FM, Suhail S, Sonam M, et al. Antifungal efficacy of herbs. J Oral Biol Craniofac Res 2019; 9(1): 28-32. doi: 10.1016/j.jobcr.2018.06.002 PMID: 30197861
  38. Eltaysh R, Rizk M, Sayed S, Abouelnasr K, Abdallah A, Igarashi I. Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites. J Adv Vet Anim Res 2022; 9(2): 267-74. doi: 10.5455/javar.2022.i592 PMID: 35891656
  39. El Ouahdani K, Es-safi I, Mechchate H, et al. Thymus algeriensis and Artemisia herba-alba Essential Oils: Chemical analysis, antioxidant potential and in vivo anti-inflammatory, analgesic activities, and Acute toxicity. Molecules 2021; 26(22): 6780. doi: 10.3390/molecules26226780 PMID: 34833872
  40. Bekka-Hadji F, Bombarda I, Djoudi F, Bakour S, Touati A. Chemical composition and synergistic potential of Mentha pulegium l. and Artemisia herba alba asso. essential oils and antibiotic against multi-drug resistant bacteria. Molecules 2022; 27(3): 1095. doi: 10.3390/molecules27031095 PMID: 35164360
  41. Ouedrhiri W, Mechchate H, Moja S, et al. Optimized antibacterial effects in a designed mixture of essential oils of Myrtus communis, Artemisia herba-alba and Thymus serpyllum for wide range of applications. Foods 2022; 11(1): 132. doi: 10.3390/foods11010132 PMID: 35010259
  42. Ratiu IA, Ligor T, Bocos-Bintintan V, Buszewski B. Mass spectrometric techniques for the analysis of volatile organic compounds emitted from bacteria. Bioanalysis 2017; 9(14): 1069-92. doi: 10.4155/bio-2017-0051 PMID: 28737423
  43. Kunze-Szikszay N, Euler M, Perl T. Identification of volatile compounds from bacteria by spectrometric methods in medicine diagnostic and other areas: Current state and perspectives. Appl Microbiol Biotechnol 2021; 105(16-17): 6245-55. doi: 10.1007/s00253-021-11469-7 PMID: 34415392
  44. Rahim ZHA, Thurairajah N. Scanning electron microscopic study of piper betle l. leaves extract effect against Streptococcus mutans ATCC 25175. J Appl Oral Sci 2011; 19(2): 137-46. doi: 10.1590/S1678-77572011000200010 PMID: 21552715
  45. Kara M, Oztas E, Ramazanoğulları R, et al. Benomyl, a benzimidazole fungicide, induces oxidative stress and apoptosis in neural cells. Toxicol Rep 2020; 7: 501-9. doi: 10.1016/j.toxrep.2020.04.001 PMID: 32337162
  46. da Cruz Cabral L, Pinto FV, Patriarca A. Application of plant derived compounds to control fungal spoilage and mycotoxin production in foods. Int J Food Microbiol 2013; 166(1): 1-14. doi: 10.1016/j.ijfoodmicro.2013.05.026 PMID: 23816820

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