Effect of cold stress on anthocyanin content and anthocyanin biosynthesis pathway gene expression in potato Solanum tuberosum L. leaves

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Cold stress has a negative effect on the ontogenesis of plants, which cold resistance is associated, among other things, with the accumulation of anthocyanins with antioxidant activity. The aim of this work was to analyze the cold stress effect on the content of anthocyanins and the regulation of anthocyanins biosynthesis in potato (Solanum tuberosum L.). In the leaves of the cv. Lady Claire plants, subjected to a two-day low-temperature exposure followed by a recovery period, the expression of the anthocyanins biosynthesis genes was determined in the dynamics of the experiment. Significant activation of regulatory (StHY5, StJAF13) and structural (StCHS2, StCHI, StF3H, StDFR) genes of the pathway was revealed at the beginning of the stress and by the end of the recovery phase. Expression of the structural gene StANS did not change. In the same leaves, the content of anthocyanins was determined and its increase was shown at the beginning and end of cold stress. Similar dynamics of gene expression (except StANS) was found to be consistent with fluctuations in anthocyanin levels. Overall, it was demonstrated that lower temperatures stimulated the expression of anthocyanin biosynthesis genes and anthocyanin accumulation in potato leaves. The data obtained may contribute to understanding the molecular mechanism of anthocyanin biosynthesis regulation in response to abiotic stresses.

作者简介

A. Bykova

Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: kulakova_97@mail.ru
Moscow, 119071 Russia

A. Meleshin

Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: kulakova_97@mail.ru
Moscow, 119071 Russia

A. Shchennikova

Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: kulakova_97@mail.ru
Moscow, 119071 Russia

E. Kochieva

Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences; Lomonosov Moscow State University, Faculty of Biology

编辑信件的主要联系方式.
Email: kulakova_97@mail.ru
Moscow, 119071 Russia; Moscow, 119991 Russia

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