The parallel comparative study of metabolism of fatty acids in erythrocytes and other tissues under experimental diabetes mellitus


The parallel study of impact of Streptozotocin diabetes mellitus on level of lipids and content of fatty acids in blood, in homogenates of skin, liver and muscular tissue in conditions of oxidative stress demonstrated that in rats at early period of development of diabetes mellitus alongside with hypertriglyceridimia and hypercholesterolimia, significant alterations are observed in content of fatty acids of blood and homogenates of analyzed tissues. The alterations are accompanied with peroxidation of lipids and decreasing of activity of enzymes-antioxidants and also decreasing of degree of utilization of glucose by erythrocytes. under experimental diabetes mellitus significant increasing of saturated fatty acids, especially palmitic fatty acid, results in decreasing of level of mono unsaturated fatty acids (at the expense of oleic fatty acids) that significantly differs of corresponding spectrum in patients with diabetes mellitus. The results of study also testify that already at early stages of development of diabetes mellitus alterations in pool of fatty acids of erythrocytes and homogenates of liver, skin and cardiac muscle similar (with slightly differences) and in all analyzed tissues disorders of fatty acids metabolism are characterized by atherosclerotic direction.

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About the authors

Nina P. Mikaelyan

Pirogov Russian national research medical university Minzdrav of Russia

117997, Moscow, Russian Federation
professor, of chair of biochemistry and molecular biology of medical facultet the N.I. Pirogov Russian national research medical university Minzdrav of Russia

Z. Kh Nguen

Pirogov Russian national research medical university Minzdrav of Russia

117997, Moscow, Russian Federation

O. S Komarov

Pirogov Russian national research medical university Minzdrav of Russia

117997, Moscow, Russian Federation

K. A Mikaelyan

Pirogov Russian national research medical university Minzdrav of Russia

117997, Moscow, Russian Federation


  1. Inzucchi S.E., Bergenstal R.M., Buse J.B., Diamant M., Ferrannini E., Nauck M. et al. Management of hyperglycemia in type 2 diabetes: a patient-centered approach: position statement of the American Diabetes Association (ADA) and the European Association for the Study of Diabetes (EASD). Diabetes Care. 2012; 35(6): 1364-79.
  2. Кроненберг Г.М., Мелмед Ш., Полонски К.С., Ларсен П.Р. Эндокринология по Вильямсу. Сахарный диабет и нарушения углеводного обмена. Перевод с английского. М.: ГЭОТАР-Медиа; 2010.
  3. Кураева Т.Л. Метформин в лечении сахарного диабета 2 типа у детей и подростков. Эффективная фармакотерапия. 2011; (26): 28-33.
  4. Петунина Н.А., Трухина Л.В., Синицына Е.И., Шестакова М.В. Глюкагон и альфа-клетки - новая терапевтическая мишень в лечении сахарного диабета. Сахарный диабет. 2013; (3): 35-40.
  5. Harris W.S., Miller M., Tighe A.P., Davidson M.H., Schaefer E.J. Omega-3 fatty acids and coronary heart disease risk: clinical and mechanistic perspectives. Atherosclerosis. 2008; 197: 12-24.
  6. Ziegler D., Sohr C.G., Nourooz-Zadeh J. O xidative stress and antioxidant defense in relation to the severity of diabetic polyneuropathy and cardiovascular autonomic neuropathy. Diabetes Care. 2004; 27(9): 2178-83.
  7. Rodríguez-Carrizalez A.D., Castellanos-González J.A., Martínez-Romero E.C., Miller-Arrevillaga G., V illa-Hernández D., Hernández-Godínez P.P. et al. O xidants, antioxidants and mitochondrial function in non-proliferative diabetic retinopathy. J. Diabetes. 2014; 6(2): 167-75.
  8. Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Med. 1976; 15(2): 212-6.
  9. Королюк М.А., Иванова Л.И., Майорова И.Г. Метод определения активности каталазы. Лабораторное дело. 1988; (1): 16-9.
  10. Folch J., Lees M., Sloane Stanley G.H. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 1957; 226(1): 497-509.
  11. Ichihara K., Fukubayashi Y. Preparation of fatty acid methyl esters for gas-liquid chromatography. J. Lipid. Res. 2010; 51(3): 635-40.
  12. Микаелян Н.П., Нгуен З.Х., Терентьев А.А. Метаболические нарушения в мембранах эритроцитов и гомогенатах печеночной ткани при сахарном диабете в эксперименте. Бюллетень экспериментальной биологии и медицины. 2015; (2): 154-8.



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